Identification of proteomic changes during differentiation of adult mouse subventricular zone progenitor cells

• Published in: Stem cells and development Vol. 16; no. 1; p. 143
• Main Authors: Salim, Kamran, Guest, Paul C, Skynner, Heather A, Bilsland, James G, Bonnert, Timothy P, McAllister, George, Munoz-Sanjuan, Ignacio
• Format: Journal Article
• Language: English
• Published: United States 01-02-2007
• Subjects: Adult Stem Cells - cytology; Adult Stem Cells - metabolism; Animals; Blotting, Western; Cell Culture Techniques; Cell Cycle; Cell Differentiation; Electrophoresis, Gel, Two-Dimensional - methods; Intercellular Signaling Peptides and Proteins - metabolism; Lateral Ventricles - cytology; Lateral Ventricles - metabolism; Metabolic Networks and Pathways; Mice; Mice, Inbred C57BL; Multipotent Stem Cells - cytology; Multipotent Stem Cells - metabolism; Nerve Tissue Proteins - chemistry; Nerve Tissue Proteins - metabolism; Neurons - cytology; Neurons - metabolism; Peptide Mapping; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 1547-3287
• DOI: 10.1089/scd.2006.00100

The use of neural precursor cells (NPCs) represents a promising repair strategy for many neurological disorders. However, the molecular events and biological features that control NPC proliferation and their differentiation into neurons, astrocytes, and oligodendrocytes are unclear. In the present study, we used a comparative proteomics approach to identify proteins that were differentially regulated in NPCs after short-term differentiation. We also used a subcellular fractionation technique for enrichment of nuclei and other dense organelles to identify proteins that were not readily detected in whole cell extracts. In total, 115 distinct proteins underwent expression changes during NPC differentiation. Forty one of these were only identified following subcellular fractionation. These included transcription factors, RNA-processing factors, cell cycle proteins, and proteins that translocate between the nucleus and cytoplasm. Biological network analysis showed that the differentiation of NPCs was associated with significant changes in cell cycle and protein synthesis machinery. Further characterization of these proteins could provide greater insight into the mechanisms involved in regulation of neurogenesis in the adult central nervous system (CNS) and potentially identify points of therapeutic intervention.