Nuclear DNA content and ultrastructure of secretory cells of Vicia faba L. stigma

• Published in: Acta Societatis Botanicorum Poloniae Vol. 63; no. 2; pp. 139 - 145
• Main Authors: Wróbel, Bogdan, Bednarska, Elżbieta
• Format: Journal Article
• Language: English
• Published: Warsaw Polish Botanical Society 01-01-1994
• Subjects: Biosynthesis; Deoxyribonucleic acid; Developmental stages; Dictyosomes; DNA; Lipids; nuclear DNA content; Nuclei; Plastids; Secretion; secretory cells; stigma; Ultrastructure; Vicia faba
• ISSN: 0001-6977; 2083-9480
• DOI: 10.5586/asbp.1994.018

The object of study was the level of nuclear DNA and the ultrastructural transformations in the secretory cells of the stigma in Vicia faba L. It has been found that the stigmal cells which are active in biogenesis and exudate secretion are diploid cells whose differentiation starts from 2C DNA level. The presence of a population of nuclei with an amount DNA of about 2.5 C suggests that the metabolic activity of those cells may be regulated through supplementary incomplete replication. The ultrastructural transformations of secretory cells point to three stages of biogenesis and secretion of exudate. Stage I, before the start of the cell's secretory functions, is characterized by the development of the protein synthesizing apparatus and the activity of dictyosomes. In development stage II vesicular electron-transparent exudate is secreted. Stage III of exudate biogenesis is production of lipids. They form mainly in the plastids and are secreted with the involvement of the cell's vacuolar system.