Absence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil

• Published in: Revista brasileira de parasitologia veterinaria Vol. 33; no. 2; p. e007924
• Main Authors: Lignon, Julia Somavilla, Pinto, Diego Moscarelli, Teles, Mariana Accorsi, Trindade, Maira Aparecida Christello, Portela, Priscila Rockenbach, Monteiro, Silvia Gonzalez, Martins, Kauê Rodriguez, Cunha, Rodrigo Casquero, Pappen, Felipe Geraldo, Bohm, Bianca Conrad, Bruhn, Fábio Raphael Pascoti
• Format: Journal Article
• Language: English
• Published: Brazil Colégio Brasileiro de Parasitologia Veterinária 01-01-2024; Colégio Brasileiro de Parasitologia Veterinaria
• Subjects: Animals; Animals, Wild - parasitology; Brazil - epidemiology; DNA, Protozoan - analysis; Leishmania - genetics; Leishmania - isolation & purification; Leishmaniasis; Mammals - parasitology; One Health; PARASITOLOGY; PCR; Polymerase Chain Reaction; protozoa; VETERINARY SCIENCES; zoonosis; Brazil; Leishmaniose; Saúde Única; protozoários; Leishmaniasis; zoonosis; protozoa; PCR; One Health; zoonose
• ISSN: 0103-846X; 1984-2961; 1984-2961
• DOI: 10.1590/S1984-29612024038

Leishmaniasis are neglected diseases transmitted by vectors that affect domestic and wild animals, including humans. Due to its incidence and lethality, this zoonosis is a worrying public health problem, making it essential to identify all links in the transmission chain. Infection of wild mammals by Leishmania spp. remains poorly understood, especially in southern Brazil. Therefore, the objective was to research, using the PCR technique, the presence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil. Carcasses of 96 animals were collected from highways in the Pelotas microregion, Rio Grande do Sul, southern Brazil and subjected to necropsies. Tissue fragments (spleen, skin, liver, kidney, heart, lung, lymph nodes, bone marrow and blood) were collected and genomic DNA was extracted. PCR protocols targeting the ITS1, kDNA and 18S genes were tested. We found no evidence of Leishmania spp. circulation in the studied population. However, epidemiological studies like this one are of great relevance, as they allow monitoring of the occurrence of pathogens and help identify possible risk areas. As these animals act as epidemiological markers for the presence of the microorganism, studies must be carried out continuously to understand whether there are sources of infection in the region.