An intelligent method based on feed-forward artificial neural network and least square support vector machine for the simultaneous spectrophotometric estimation of anti hepatitis C virus drugs in pharmaceutical formulation and biological fluid

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 263; p. 120190
• Main Authors: Keyvan, Kiarash, Sohrabi, Mahmoud Reza, Motiee, Fereshteh
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15-12-2021
• Subjects: Artificial neural network; Daclatasvir; Least square support vector machine; Sofosbuvir; Spectrophotometry; Daclatasvir; Least square support vector machine; Artificial neural network; Spectrophotometry; Sofosbuvir
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2021.120190

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•Intelligent methods, including FF-ANN and LS-SVM were proposed.•Simultaneous spectrophotometry analysis of sofosbuvir and daclatasvir was done via these methods.•Rapid, simple, inexpensive, and accurate are the advantages of these suggested procedures.•HPLC technique was implemented to compare with spectrophotometry method.
This study proposed simple and reliable spectrophotometry method for simultaneous analysis of hepatitis C antiviral binary mixture containing sofosbuvir (SOF) and daclatasvir (DAC). This technique is based on the use of feed-forward artificial neural network (FF-ANN) and least square support vector machine (LS-SVM). FF-NN with Levenberg–Marquardt (LM) and Cartesian genetic programming (CGP) algorithms was trained to determine the best number of hidden layers and the number of neurons. This comparison demonstrated that the LM algorithm had the minimum mean square error (MSE) for SOF (1.59 × 10−28) and DAC (4.71 × 10−28). In LS-SVM model, the optimum regularization parameter (γ) and width of the function (σ) were achieved with root mean square error (RMSE) of 0.9355 and 0.2641 for SOF and DAC, respectively. The coefficient of determination (R2) value of mixtures containing SOF and DAC was 0.996 and 0.997, respectively. The percentage recovery values were in the range of 94.03–104.58 and 94.04–106.41 for SOF and DAC, respectively. Statistical test (ANOVA) was implemented to compare high-performance liquid chromatography (HPLC) and spectrophotometry, which showed no significant difference. These results indicate that the proposed method possesses great potential ability for prediction of concentration of components in pharmaceutical formulations.