14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity

• Published in: Journal of materials science. Materials in medicine Vol. 30; no. 9; pp. 99 - 4
• Main Authors: Rivero, G., Aldana, A. A., Frontini Lopez, Y. R., Liverani, L., Boccacini, A. R., Bustos, D. M., Abraham, G. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-09-2019; Springer Nature B.V
• Subjects: 14-3-3 protein; 14-3-3 Proteins - chemistry; 14-3-3 Proteins - pharmacology; Alkaline phosphatase; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Biomimetics; Bone grafts; Bone growth; Bones; Cell differentiation; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cells, Cultured; Ceramics; Chemistry and Materials Science; Composites; Differentiation (biology); Durapatite - chemistry; Durapatite - pharmacology; Electroplating - methods; Flow cytometry; Glass; Grafts; Humans; Hydroxyapatite; Materials Science; Materials Testing; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - physiology; Mesenchyme; Nanofibers - chemistry; Natural Materials; Osseointegration; Osteoblasts; Osteoblasts - drug effects; Osteoblasts - physiology; Osteogenesis - drug effects; Osteogenesis - physiology; Polyesters - chemistry; Polyesters - pharmacology; Polymer Sciences; Proteins; Regeneration; Regenerative Medicine/Tissue Engineering; Scaffolds; Stem cells; Subcutaneous Fat, Abdominal - cytology; Substitute bone; Surface Properties - drug effects; Surfaces and Interfaces; Surgical implants; Thin Films; Tissue engineering; Tissue Engineering - methods; Tissue Engineering Constructs and Cell Substrates; Tissue Scaffolds - chemistry; Viability
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-019-6302-2

Adipose-derived mesenchymal stem cells (ASCs) accelerate the osteointegration of bone grafts and improve the efficiency in the formation of uniform bone tissue, providing a practical and clinically attractive approach in bone tissue regeneration. In this work, the effect of nanofibrous biomimetic matrices composed of poly(ε-caprolactone) (PCL), nanometric hydroxyapatite (nHA) particles and 14-3-3 protein isoform epsilon on the initial stages of human ASCs (hASCs) osteogenic differentiation was investigated. The cells were characterized by flow cytometry and induction to differentiation to adipogenic and osteogenic lineages. The isolated hASCs were induced to differentiate to osteoblasts over all scaffolds, and adhesion and viability of the hASCs were found to be similar. However, the activity of alkaline phosphatase (ALP) as early osteogenic marker in the PCL-nHA/protein scaffold was four times higher than in PCL-nHA and more than five times than the measured in neat PCL.