Simultaneous determination of phenylbutazone and its metabolites in plasma and urine by high-performance liquid chromatography

• Published in: Journal of chromatography Vol. 183; no. 3; p. 331
• Main Authors: Marunaka, T, Shibata, T, Minami, Y, Umeno, Y
• Format: Journal Article
• Language: English
• Published: Netherlands 01-01-1980
• Subjects: Animals; Chromatography, High Pressure Liquid - methods; Dogs; Gas Chromatography-Mass Spectrometry; Humans; Kinetics; Male; Oxyphenbutazone - blood; Oxyphenbutazone - urine; Phenylbutazone - analogs & derivatives; Phenylbutazone - blood; Phenylbutazone - urine; Rats
• DOI: 10.1016/S0378-4347(00)81713-2

A high-performance liquid chromatographic method was developed for the simultaneous determination of phenylbutazone and its metabolites, oxyphenbutazone and gamma-hydroxy-phenylbutazone, in plasma and urine. Samples were acidified with hydrochloric acid and extracted with benzene--cyclohexane (1:1, v/v). The extract was redissolved in methanol and chromatographed on a muBondapak C18 column using a mobile phase of methanol--0.01 M sodium acetate buffer (pH 4.0) in a linear gradient (50 to 100% methanol at 5% min; flow-rate 2.0 ml/min) in a high-performance liquid chromatograph equipped with an ultraviolet absorbance detector (254 nm). The detection limit for phenylbutazone, oxyphenbutazone and for gamma-hydroxyphenylbutazone was 0.05 microgram/ml. A precise and sensitive assay for the determination of phenylbutazone and its metabolites was established.