AKR1B10 protects against UVC-induced DNA damage in breast cancer cells

• Published in: Acta biochimica et biophysica Sinica Vol. 53; no. 6; pp. 726 - 738
• Main Authors: Zeng, Yuanqing, Li, Jia, Guo, Wangyuan, Luo, Weihao, Liu, Xiangting, He, Rongzhang, Hu, Zheng, Duan, Lili, Xia, Chenglai, Luo, Dixian
• Format: Journal Article
• Language: English
• Published: China 01-06-2021
• Subjects: NF-κB; AKR1B10; UVC; DNA damage; cell cycle checkpoint
• ISSN: 1672-9145; 1745-7270
• DOI: 10.1093/abbs/gmab045

The cellular response to DNA damage is crucial for maintaining the integrity and stability of molecular structure. To maintain genome stability, DNA-damaged cells should be arrested so that mutations can be repaired before replication. Although several key components required for this arrest have been discovered, the majority of the pathways are still unclear. Through a number of assays, including cell viability, colony formation, and apotheosis assay, we found that AKR1B10 protected cells from UVC-induced DNA damage. Surprisingly, UVC-induced γH2AX foci and DNA double-strand breaks in the AKR1B10-overexpressing cells were ∼4-5 folds lower than those in the control group. The expression levels of AKR1B10, p53, chk1, chk2, nuclear factor (NF)-κB, and p65 showed dynamic changes in response to UVC irradiation. Our results suggested that AKR1B10 is involved in the pathway of cell cycle checkpoint and NF-κB in DNA damage. Taken together, our results suggest that AKR1B10 is involved in the repair of the DNA double-strand break, which provides a new insight into the role of AKR1B10 in DNA damage repair and indicates a new trail in tumorigenesis and cancer drug resistance.