A set of near-isogenic lines for blast resistance genes with an Indica-type rainfed lowland elite rice ( Oryza sativa L.) genetic background

• Published in: Field crops research Vol. 123; no. 1; pp. 19 - 27
• Main Authors: Koide, Yohei, Ebron, Leodegario A., Kato, Hiroshi, Tsunematsu, Hiroshi, Telebanco-Yanoria, Mary Jeanie, Kobayashi, Nobuya, Yokoo, Masao, Maruyama, Sachio, Imbe, Tokio, Fukuta, Yoshimichi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 18-07-2011
• Subjects: Blast ( Pyricularia grisea (Cooke) Sacc.); Multiline variety; Near-isogenic line; Oryza sativa; Resistance gene; Rice ( Oryza sativa L.); Philippines; Blast ( Pyricularia grisea (Cooke) Sacc.); Resistance gene; Multiline variety; Rice ( Oryza sativa L.); Near-isogenic line
• ISSN: 0378-4290; 1872-6852
• DOI: 10.1016/j.fcr.2011.04.005

► Near-isogenic lines (NILs) for blast resistance genes were developed. ► They have a genetic background of an Indica-type rice variety. ► The introgression of resistance genes was confirmed by reactions to blast. ► They could be used as a multiline variety in the rainfed lowland in the tropics.
A set of near-isogenic lines for blast resistance genes was developed by using an Indica-type elite rice variety, IR49830-7-1-2-2, suitable for the rainfed lowland conditions in the tropics, as a genetic background. Initially, we revealed that IR49830-7-1-2-2 harbors five blast resistance genes –
Pia,
Pib,
Pik-s,
Pita, and
Pi11(t) – by using a differential system involving 19 selected standard blast isolates from the Philippines. Based on this result, we developed nine near-isogenic lines (NILs) targeting eight resistance genes –
Pik,
Pi7(t),
Pi3,
Pi5,
Pita-2,
Piz-5,
Pish, and
Pi9 – by recurrent backcrossing. The introgression of each resistance gene in the NILs was confirmed by reaction patterns to the blast isolates, allelism tests, and DNA marker analysis. In addition, a genome-wide DNA marker survey revealed that most of the chromosome regions in each NIL were of the IR49830-7-1-2-2 type. The agricultural characteristics of most of the developed NILs were almost the same as those of IR49830-7-1-2-2. Moreover, with one exception, they showed submergence tolerance similar to IR49830-7-1-2-2. The developed NILs could be used as a multiline variety suitable for the rainfed lowland in the tropics.