An in Vitro Investigation of the Intracellular Bioactivity of Amoxicillin, Clindamycin, and Erythromycin for Staphylococcus aureus

• Published in: The Journal of infectious diseases Vol. 153; no. 3; pp. 593 - 600
• Main Authors: Anderson, R., Joone, G., van Rensburg, C. E. J.
• Format: Journal Article
• Language: English
• Published: Chicago, IL Oxford University Press 01-03-1986; University of Chicago Press
• Subjects: Amoxicillin - pharmacology; Antibacterial agents; Antibiotics; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antimicrobials; Bacteria; Biological and medical sciences; Chemiluminescence; Chronic granulomatous disease; Clindamycin - pharmacology; Erythromycin - pharmacology; Fluorescence; Fluorometry; Humans; Medical sciences; Methods; Microbial Sensitivity Tests; Neutrophils - drug effects; Neutrophils - microbiology; Original Articles; Pathogens; Phagocytes; Phagocytosis; Pharmacology. Drug treatments; Sodium Fluoride - pharmacology; Staphylococcus aureus - drug effects; Viability; Staphylococcus aureus; Granulocyte; Erythromycin; In vitro; Macrolide; Biological activity; Penicillin derivatives; Antibiotic; Bacteria; Micrococcales; Micrococcaceae; Intracellular; Antibacterial agent; Staphylococcus aureus
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/153.3.593

The intraphagocytic bioactivities for Staphylococcus aureus of amoxicillin, clindamycin, and erythromycin (0.0075-20 µ/ml) were measured in human polymorphonuclear leukocytes (PMNLs) with the combination of a fluorochrome microassay and a radioassay. PMNLs with normal or depleted membrane-associated oxidative metabolism were used to investigate the interactions that may occur between the intrinsic O2 dependent antimicrobial systems of human phagocytes and antimicrobial agents in the elimination of intracellular microbial pathogens. Neutralization of 02-dependent antimicrobial systems with retention of phagocytic capacity was achieved with use of PMNLs.from four children with chronic granulomatous disease (COD) or NaF-pulsed normalPMNLs. None of the test antibiotics possessed intracellular bactericidal activity. Clindamycin and erythromycin possessed significant intracellular bacteriostatic activity relativ~ to the modest activity of amoxicillin. Optimal intracellular bioactivity of all three antibiotics was obtained with normal PMNLs relative to NaF-pulsed or COD PMNLs, a result indicating the existence of beneficial interactions between the antimicrobial agents and the O2 dependent antimicrobial systems of PMNLs.