Fidelity of enzymatic ligation for DNA computing

We describe a convenient assay for rapid qualitative evaluation of hybridization/ligation fidelity. The approach uses randomized probe strands of DNA and restriction enzyme digestion after amplification of reaction products by the polymerase chain reaction (PCR). We report ligation efficiencies and...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of computational biology Vol. 7; no. 6; p. 839
Main Authors:	Faulhammer, D, Lipton, R J, Landweber, L F
Format:	Journal Article
Language:	English
Published:	United States 01-01-2000
Subjects:	DNA - chemistry DNA - metabolism DNA Ligases - chemistry DNA Ligases - metabolism Enzymes - chemistry Enzymes - metabolism Models, Biological Molecular Biology - methods Polymerase Chain Reaction - methods Taq Polymerase - chemistry Taq Polymerase - metabolism
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We describe a convenient assay for rapid qualitative evaluation of hybridization/ligation fidelity. The approach uses randomized probe strands of DNA and restriction enzyme digestion after amplification of reaction products by the polymerase chain reaction (PCR). We report ligation efficiencies and fidelities of two DNA ligases, T4 DNA ligase and Thermus aquaticus (Taq) DNA ligase, over a range of temperatures.
ISSN:	1066-5277
DOI:	10.1089/10665270050514963