Transgenic assessment of CFP-mediated cercosporin export and resistance in a cercosporin-sensitive fungus

• Published in: Current genetics Vol. 41; no. 1; pp. 25 - 30
• Main Authors: Upchurch, Robert G, Rose, Mark S, Eweida, Mohamed, Callahan, Terrence M
• Format: Journal Article
• Language: English
• Published: United States 01-04-2002
• Subjects: Antifungal Agents - pharmacology; Ascomycota - genetics; Ascomycota - metabolism; Carrier Proteins - genetics; Carrier Proteins - metabolism; Drug Resistance, Fungal - genetics; Fungal Proteins; Membrane Transport Proteins; Organisms, Genetically Modified; Perylene - analogs & derivatives; Perylene - pharmacology
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/s00294-002-0280-4

Cercosporin is a toxic polyketide produced by many phytopathogenic members of the fungal genus Cercospora. Cercospora species, themselves, exhibit the highest level of self-resistance to this almost universally toxic photosensitizer. Although the mechanism of cercosporin self-resistance is multi-faceted, partial resistance does appear to be provided by the encoded product of CFP ( cercosporin facilitator protein), a gene recently isolated from the pathogen of soybean, C. kikuchii. CFP has significant similarity to the major facilitator superfamily of integral membrane transport proteins. We expressed CFP in the cercosporin non-producing, cercosporin-sensitive fungus, Cochliobolus heterostrophus, in order to assess the transport activity of CFP and the contribution of CFP to cercosporin resistance in a fungal species free of endogenous toxin production. Expression of the CFP transgene in this fungus results in increased resistance to cercosporin due, apparently, to its export out of the fungus.