Haemorrheology of equine platelet-neutrophil aggregates

• Published in: Comparative Haematology International Vol. 9; no. 2; pp. 55 - 59
• Main Authors: Weiss, D. J., Evanson, O. A., McClenahan, D., Fagliari, J. J.
• Format: Journal Article
• Language: English
• Published: London Springer Nature B.V 01-01-1999
• Subjects: Aggregates
• ISSN: 0938-7714; 1618-5641; 1618-565X
• DOI: 10.1007/BF02585536

The filterability of platelet-neutrophil aggregates through filters containing 5 μm pores was determined to evaluate the potential of plateletneutrophil aggregates to alter blood flow in the microvasculature. Incubation of platelet-neutrophil mixture with platelet-activating factor (PAF) and with 0.5 μM adenosine diphosphate (ADP) resulted in large platelet-neutrophil aggregates which rapidly plugged filter pores. Incubation of platelet-neutrophil mixtures with lower concentrations of ADP resulted in small platelet-neutrophil aggregates that contained only one neutrophil. Filtration of these samples resulted in a 1.3-, 3.2- and 3.7-fold increase in initial filtration pressure at ADP concentrations of 0.05, 0.15 and 0.25 μM, respectively. We conclude that platelet-neutrophil aggregates have reduced deformability and may alter blood flow in the microvasculature.[PUBLICATION ABSTRACT]