Enzymatic synthesis of citronellol laurate in organic media and in supercritical carbon dioxide

• Published in: Biochemical engineering journal Vol. 42; no. 1; pp. 6 - 12
• Main Authors: Habulin, Maja, Šabeder, Saša, Sampedro, Miguel Acebes, Knez, Željko
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15-10-2008; Amsterdam Elsevier Science; New York, NY
• Subjects: Biocatalysis; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Citronellol laurate; Food engineering; Fundamental and applied biological sciences. Psychology; Immobilized enzymes; Lipase; Methods. Procedures. Technologies; Supercritical carbon dioxide; Biocatalysis; Immobilized enzymes; Supercritical carbon dioxide; Lipase; Citronellol laurate; Food engineering; Enzymatic synthesis; Organic solvent; Citronellol laurate;Biocatalysis;Immobilized enzymes;Lipase;Food engineering;Supercritical carbon dioxide; Supercritical state; Carbon dioxide
• ISSN: 1369-703X; 1873-295X
• DOI: 10.1016/j.bej.2008.05.012

Short-chain esters of carboxylic acids and monoterpene alcohols are very important compounds in food, cosmetic and pharmaceutical industries. Lipase-catalyzed esterification of β-citronellol and lauric acid was performed in organic solvents and in supercritical carbon dioxide (SC CO
2). The optimal conditions found in organic solvents at atmospheric pressure were: an equimolar ratio of substrates, a temperature of 60
°C, an agitation speed of 500
rpm, 12% (w/w of substrates) enzyme preparation of lipase B from
Candida antarctica. The highest conversion in SC CO
2 was obtained at 60
°C and 10
MPa with ethyl methylketone serving as a co-solvent.