Polyglycerol-bound phosphotriesterase enzyme model complexes for detection and hydrolysis of phosphorus species in aqueous solution

• Published in: Tetrahedron Vol. 65; no. 22; pp. 4298 - 4303
• Main Authors: Mangalum, Anshuman, Smith, Rhett C.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 30-05-2009; Elsevier
• Subjects: Chemistry; Exact sciences and technology; Heterocyclic compounds; Heterocyclic compounds with only one n hetero atom and condensed derivatives; Hyperbranched polymer; Organic chemistry; Phosphohydrolase; Preparations and properties; Pyrophosphate sensor; Phosphohydrolase; Hyperbranched polymer; Pyrophosphate sensor; Nitrogen heterocycle; Ligand; Metal; Phosphorus Organic compounds; Pyridine derivatives; Amine; Water solubility; Diphosphates; Biological receptor; Phosphohydrolases; Chemical solution; Branched polymer; Measurement sensor; Enzyme model; Buffer system; Reaction rate; Hydrolysis; Toxin; Neutral medium; Polynuclear complex; Organic phosphate; Organic phosphorus compounds; Aqueous solution; Detection
• ISSN: 0040-4020; 1464-5416
• DOI: 10.1016/j.tet.2009.03.051

Phosphotriesterase models incorporating di(2-picolyl)amino ligands supported by
m-xylylene or 2-hydroxy-
m-xylylene scaffolds have been tethered to the periphery of a water-soluble hyperbranched polyglycerol (PG). In aqueous solution buffered at pH 7.4, the polymeric complexes of Zn
2+ are useful receptors for polymeric indicator displacement assays for phosphate and pyrophosphate employing commercial complexometric indicators. Under the same conditions, the Co
3+ effectively hydrolyze
p-nitrophenylphosphate with approximately five orders of magnitude rate enhancement versus uncatalyzed hydrolysis. These systems offer promising results as mixed-metal dual detect-decontaminate materials for organophosphorus toxins under mild, neutral aqueous conditions.
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