Selective enrichment of commensal gut bacteria protects against Citrobacter rodentium-induced colitis

• Published in: American journal of physiology: Gastrointestinal and liver physiology Vol. 309; no. 3; p. G181
• Main Authors: Vong, Linda, Pinnell, Lee J, Määttänen, Pekka, Yeung, C William, Lurz, Eberhard, Sherman, Philip M
• Format: Journal Article
• Language: English
• Published: United States 01-08-2015
• Subjects: Actinobacteria - physiology; Animals; Bacteriological Techniques; Citrobacter rodentium - physiology; Disease Models, Animal; Dysbiosis - immunology; Dysbiosis - prevention & control; Enterobacteriaceae Infections - microbiology; Enterobacteriaceae Infections - prevention & control; Gammaproteobacteria - physiology; Host-Pathogen Interactions - immunology; Intestinal Mucosa - immunology; Lactobacillus - physiology; Mice; Mice, Inbred C57BL; Microbial Interactions; Microbiota; lactobacilli; colitis; neutrophil extracellular traps; Citrobacter rodentium; gut microbiota
• ISSN: 1522-1547
• DOI: 10.1152/ajpgi.00053.2015

The intestinal microbiota plays a key role in shaping the host immune system. Perturbation of gut microbial composition, termed dysbiosis, is associated with an increased susceptibility to intestinal pathogens and is a hallmark of a number of inflammatory, metabolic, and infectious diseases. The prospect of mining the commensal gut microbiota for bacterial strains that can impact immune function represents an attractive strategy to counteract dysbiosis and resulting disease. In this study, we show that selective enrichment of commensal gut lactobacilli protects against the murine pathogen Citrobacter rodentium, a well-characterized model of enteropathogenic and enterohemorrhagic Escherichia coli infection. The lactobacilli-enriched bacterial culture prevented the expansion of Gammaproteobacteria and Actinobacteria and was associated with improved indexes of epithelial barrier function (dextran flux), transmissible crypt hyperplasia, and tissue inflammatory cytokine levels. Moreover, cultivation of gut bacteria from Citrobacter rodentium-infected mice reveals the differential capacity of bacterial subsets to mobilize neutrophil oxidative burst and initiate the formation of weblike neutrophil extracellular traps. Our findings highlight the beneficial effects of a lactobacilli-enriched commensal gut microenvironment and, in the context of an intestinal barrier breach, the ability of neutrophils to immobilize both commensal and pathogenic bacteria.