Identification of Treponema hyodysenteriae and Treponema innocens using two four-hour identification systems

• Published in: Journal of veterinary diagnostic investigation Vol. 3; no. 3; p. 211
• Main Authors: Achacha, M, Messier, S
• Format: Journal Article
• Language: English
• Published: United States 01-07-1991
• Subjects: Animals; Reagent Kits, Diagnostic - veterinary; Swine; Swine Diseases - microbiology; Treponema - isolation & purification; Treponemal Infections - microbiology; Treponemal Infections - veterinary
• ISSN: 1040-6387
• DOI: 10.1177/104063879100300304

Two 4-hour identification systems, the RapID-ANAII (Innovative Diagnostic Systems) and the ANI card (Vitek Systems), were used to identify isolates of Treponema hyodysenteriae and T. innocens. Twenty-one isolates of T. innocens and 53 isolates of T. hyodysenteriae were tested with both systems. With the ANI system, alpha-galactosidase was the only test that differentiated the two species. With the RapID-ANAII system, alpha-galactosidase and indole tests allowed differentiation of the two species. Treponema hyodysenteriae was alpha-galactosidase negative and indole positive, whereas T. innocens produces the opposite reactions. Three isolates were alpha-galactosidase negative and indole negative. These isolates represent a group intermediate between the two officially described species. The two species of swine Treponema can be identified by commercial identification kits and a third group of isolates intermediate between the two species was identified.