Effects of Phomopsidione on the Viability, Virulence, and Metabolites Profile of Methicillin-Resistant Staphylococcus aureus (MRSA)

• Published in: Current microbiology Vol. 81; no. 4; p. 108
• Main Authors: Wei, Yee-Min, Tong, Woei-Yenn, Tan, Joo-Shun, Lim, Vuanghao, Leong, Chean-Ring, Tan, Wen-Nee
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-04-2024; Springer Nature B.V
• Subjects: Antibiotics; Bacteria; Bioengineering; Biomedical and Life Sciences; Biotechnology; Catalase; Chromatography; Discriminant analysis; Drug resistance; Extracellular polymers; Gram-positive bacteria; Life Sciences; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolites; Metabolomics; Methicillin; Microbiology; Microorganisms; Minimum inhibitory concentration; Multidrug resistance; Multidrug resistant organisms; Scientific imaging; Staphylococcus aureus; Staphylococcus infections; Virulence; Virulence factors
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-024-03627-7

Methicillin-resistant
Staphylococcus aureus
(MRSA) infections have become one of the most threatening multidrug-resistant pathogens. Thus, an ongoing search for anti-MRSA compounds remains an urgent need to effectively treating MRSA infections. Phomopsidione, a novel antibiotic isolated from
Diaporthe fraxini
, has previously demonstrated potent anti-candidal activity. The present study aimed to investigate the effects of phomopsidione on the viability, virulence, and metabolites profile of MRSA. MRSA was sensitive to phomopsidione in a concentration-dependent manner. Phomopsidione exhibited minimum inhibitory concentration and minimum bactericidal concentration of 62.5 and 500.00 µg/mL against MRSA on broth microdilution assay. The compound showed significant reduction in virulence factors production including extracellular polymeric substances quantification, catalase, and lipase. An untargeted metabolomics analysis using liquid chromatography-high resolution mass spectrometry revealed a significant difference in the metabolites profile of MRSA with 13 putatively identified discriminant metabolites. The present study suggested the potential of phomopsidione as a promising anti-MRSA agent.