Patterns of evolution in MHC class II DQA and DQB exon 2 genes of Alpine mountain hares, Lepus timidus varronis, and sympatric and parapatric brown hares, L. europaeus, from Switzerland

• Published in: Immunogenetics (New York) Vol. 76; no. 1; pp. 37 - 50
• Main Authors: Awadi, A., Ben Slimen, H., Smith, S., Makni, M., Suchentrunk, F.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-02-2024; Springer Nature B.V
• Subjects: Alleles; Allergology; Animals; Balancing; Biomedical and Life Sciences; Biomedicine; Cell Biology; Differentiation; Evolution; Evolutionary genetics; Exons; Gene flow; Gene frequency; Gene Function; Genes; Genetic diversity; Hares - genetics; Human Genetics; Hybridization; Immunology; Mountains; Natural populations; Original Article; Pathogens; Phylogeny; Polymorphism; Polymorphism, Genetic; Population studies; Populations; Positive selection; Species; Stochasticity; Switzerland; Sympatric populations; Switzerland; Hares; DQB; Introgression; DQA; Switzerland; Positive selection
• ISSN: 0093-7711; 1432-1211
• DOI: 10.1007/s00251-023-01328-2

In natural populations, hybridization is known to occur between a wide range of species. However, its evolutionary significance is less clear. Genes involved in fighting pathogens are considered excellent candidates for studying adaptive introgression, although both introgression and balancing selection can generate similar patterns of diversity and differentiation. Here, we compared
DQA
and
DQB
MHC class II and microsatellite allelic diversity of sympatric and parapatric mountain (
Lepus timidus
) and brown hare (
L. europaeus
) populations from Switzerland. We detected higher genetic diversity in brown hares compared to mountain hares at both MHC and microsatellite loci. We consider the observed patterns of microsatellite diversity both for
L. europaeus
and
L. timidus
as result of stochastic demographic processes while the pattern of MHC polymorphism of the studied hare populations can be explained by pathogen-driven selection. Rare bidirectional gene flow between both hare species seems to occur specifically for MHC alleles. However, the high number of shared alleles showing similar high frequency in both species suggests that reciprocally exchanged MHC alleles are being maintained via balancing selection. Adaptation to similar pathogen communities can also lead to parallel selection of MHC alleles. Positive selection, recombination and mutations have played different roles in shaping the patterns of MHC allelic diversity in and differentiation between both species. Results for the latter evolutionary forces do not show a better matching between the sympatric populations compared to the parapatric ones, suggesting a minor role of introgression for the observed evolutionary patterns of the studied hare species.