Fictibacillus fluitans sp. nov., isolated from freshwater pond

• Published in: Archives of microbiology Vol. 206; no. 2; p. 70
• Main Authors: Yadav, Amit, Maurya, Akanksha, Bhavsar, Yash, Teware, Ruchi, Bhatt, Agrima, DaCosta, Elaine, Thorat, Vipool, Kirdat, Kiran, Dastager, Syed G.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-02-2024; Springer Nature B.V
• Subjects: Amino acids; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Cell Biology; Deoxyribonucleic acid; DNA; Ecology; Fresh Water; Genomes; Hybridization; Life Sciences; Lipids; Microbial Ecology; Microbiology; New species; Original Paper; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogenetics; Phylogeny; Ponds; RNA, Ribosomal, 16S - genetics; rRNA 16S; Siderophores; Sodium chloride; Freshwater bacteria; Fictibacillus; Bacillus
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-023-03794-4

A Gram-positive, aerobic, rod-shaped, spore-forming bacterium, designated NE201
T
, was isolated from a freshwater pond in Village Nerur, India. Growth was observed in the range of 15–45 °C temperature with optimum at 30 °C, pH range of 5–9 (optimum at 7.0), and at concentrations of NaCl ranging between 0 and 14% (optimum 0%, w/v). The 16S rRNA gene sequence showed the highest similarity with
Fictibacillus enclensis
NIO-1003
T
(JF893461) at 99.01% followed by
F. rigui
WPCB074
T
(EU939689) at 98.9% and
F. solisalsi
CGMCC 1.6854
T
(EU046268) at 98.66%. The digital DNA–DNA hybridization (dDDH) and orthoANI values for strain NE201
T
against
F. enclensis
NIO-1003
T
(GCA_900094955.1) were 33.7% and 87.68%, respectively. The phylogenetic analysis based on the 16S rRNA gene, 92 core genes derived from the genome, and 20 proteins involving over 20,236 amino acid positions revealed the distinct phylogenetic position of strain NE201
T
and the formation of a clearly defined monophyletic clade with
F. enclensis
. The strain NE201
T
showed a unique carbon utilization and assimilation pattern that differentiated it from
F. enclensis
NIO-1003
T
. The major fatty acids were anteiso -C
15:0
(51.42%) and iso-C
15:0
(18.88%). The major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE, and diphosphatidylglycerol (DPG). The antiSMASH analyzed genome of NE201
T
highlighted its diverse biosynthetic potential, unveiling regions associated with terpene, non-ribosomal peptide synthetases (NRPS), lassopeptides, NI-siderophores, lanthipeptides (LAP), and Type 3 Polyketide Synthases (T3PKS). The overall phenotypic, genotypic, and chemotaxonomic characters strongly suggested that the strain NE201
T
represents a novel species of genus
Fictibacillus
for which the name
Fictibacillus fluitans
sp. nov. is proposed. The type strain is NE201
T
 (= MCC 5285 = JCM 36474).