New approaches concerning the testis of Astyanax lacustris (Characidae): immunohistochemical studies

• Published in: Fish physiology and biochemistry Vol. 49; no. 3; pp. 543 - 556
• Main Authors: Costa, Fabiano Gonçalves, Gomes, Chayrra Chehade, Adolfi, Mateus Contar, da Cruz Gallo de Carvalho, Mayra Costa, Zanoni, Marco Antônio, Seiva, Fábio Rodrigues Ferreira, Borella, Maria Inês
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-06-2023; Springer Nature B.V
• Subjects: Actin; Actins; Androgen receptors; Androgens; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Astyanax lacustris; Biology; Biomedical and Life Sciences; Characidae; Claudin-1 - analysis; Collagen; Collagen Type I; Cytokeratin; Cytoskeleton; Distribution; Endothelium; Epithelium; Extracellular matrix; Fibronectin; Fibronectins - analysis; Fish; Freshwater & Marine Ecology; Histology; Keratins - analysis; Labeling; Laminin; Laminin - analysis; Life Sciences; Localization; Male; Morphology; Receptors; Receptors, Androgen - analysis; Reproductive cycle; Sertoli cells; Spermatogonia; Testis; Zoology; Teleost; Extracellular matrix components; Cytoskeleton; Androgen receptor
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-023-01194-5

Astyanax lacustris
, locally known as lambari-do-rabo-amarelo, is a study model for Neotropical fish. Testis of
A. lacustris
shows deep morphophysiological changes throughout the annual reproductive cycle. This work analyzed the distribution of claudin-1, actin, and cytokeratin as elements of the cytoskeleton in germinal epithelium and interstitium; the distribution of type I collagen, fibronectin, and laminin as extracellular matrix compounds; and the localization of androgen receptor in the testis of this species. Claudin-1, cytokeratin, and actin were present in the Sertoli cells and modified Sertoli cells, and actin was also detected in peritubular myoid cells. Type I collagen were in the interstitial tissue, laminin in the basement membrane of germinal epithelium and endothelium, but fibronectin was additionally detected in the germinal epithelium compartment. The labeling of androgen receptor was higher in peritubular myoid cells and undifferentiated spermatogonia, and weaker labeling was detected in type B spermatogonia. Therefore, the present work highlights new aspects of the biology of the testis of
A. lacustris
, and contribute to amplify the understanding of this organ.