Flavobacterium psychrotrophum sp. nov. and Flavobacterium panacagri sp. nov., Isolated from Freshwater and Soil

• Published in: The journal of microbiology Vol. 61; no. 10; pp. 891 - 901
• Main Authors: Kim, Yong-Seok, Hwang, Eun-Mi, Jeong, Chang-Myeong, Cha, Chang-Jun
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01-10-2023; Springer Nature B.V
• Subjects: Bacterial Typing Techniques; Biomedical and Life Sciences; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - analysis; Flavobacterium; Fresh Water - microbiology; Freshwater; Gene sequencing; Genomic analysis; Gliding; Hybridization; Inland water environment; Life Sciences; Lipids; Menaquinones; Microbial Systematics and Evolutionary Microbiology; Microbiological strains; Microbiology; New species; Nucleotide sequence; Nucleotides; Phosphatidylethanolamine; Phylogenetics; Phylogeny; Pigments; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Soil; Soil microorganisms; Soils; Strains (organisms); Vitamin K 2; Novel species; Genome; Flavobacterium panacagri; Flavobacterium; Flavobacterium psychrotrophum
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-023-00081-1

Two novel bacterial strains CJ74
T
and CJ75
T
belonging to the genus
Flavobacterium
were isolated from freshwater of Han River and ginseng soil, South Korea, respectively. Strain CJ74
T
was Gram-stain-negative, aerobic, rod-shaped, non-motile, and non-flagellated, and did not produce flexirubin-type pigments. Strain CJ75
T
was Gram-stain-negative, aerobic, rod-shaped, motile by gliding, and non-flagellated, and produced flexirubin-type pigments. Both strains were shown to grow optimally at 30 °C in the absence of NaCl on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CJ74
T
and CJ75
T
belonged to the genus
Flavobacterium
and were most closely related to
Flavobacterium niveum
TAPW14
T
and
Flavobacterium foetidum
CJ42
T
with 96.17% and 97.29% 16S rRNA sequence similarities, respectively. Genomic analyses including the reconstruction of phylogenomic tree, average nucleotide identity, and digital DNA-DNA hybridization suggested that they were novel species of the genus
Flavobacterium
. Both strains contained menaquinone 6 (MK-6) as the primary respiratory quinone and phosphatidylethanolamine as a major polar lipid. The predominant fatty acids of both strains were iso-C
15:0
and summed feature 3 (C
16:1
ω7c
and/or C
16:1
ω6c
). Based on the polyphasic taxonomic study, strains CJ74
T
and CJ75
T
represent novel species of the genus
Flavobacterium
, for which names
Flavobacterium psychrotrophum
sp. nov. and
Flavobacterium panacagri
sp. nov. are proposed, respectively. The type strains are CJ74
T
(=KACC 19819
T
 =JCM 32889
T
) and CJ75
T
(=KACC 23149
T
 =JCM 36132
T
).