Effect of conA-unbound proteins from Melipona beecheii honey on the formation of Pseudomonas aeruginosa ATCC 27853 biofilm

• Published in: Archives of microbiology Vol. 206; no. 1; p. 54
• Main Authors: Pool-Yam, Luis, Ramón-Sierra, Jesús, Oliva, A. I., Zamora-Bustillos, Roberto, Ortiz-Vázquez, Elizabeth
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-01-2024; Springer Nature B.V
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Antimicrobial agents; Archives & records; Bacteria; Bees; Biochemistry; Biofilms; Biomedical and Life Sciences; Biotechnology; Cell Biology; Concanavalin A; Defense mechanisms; Drug resistance; Ecology; Genes; Honey; Life Sciences; Mannose; Melipona beecheii; Microbial Ecology; Microbiology; Nosocomial infections; Original Paper; Pathogens; Proteins; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas Infections; ConA-unbound protein fraction; Stingless bee; Antibiofilm; Pseudomonas aeruginosa; Melipona beecheii
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-023-03783-7

Pseudomonas aeruginosa
is an opportunistic bacterium that can form a biofilm with the ability to colonize different surfaces and for increasing resistance to antibiotics. An alternative to solve this problem may be the use of non-glucose/mannose glycosylated proteins from
Melipona beecheii
honey, which are capable of inhibiting the growth of this pathogen. In this work, the antibiofilm activity of the conA-unbound protein fraction (F1) from
M. beecheii
was evaluated. The crude protein extract (CPE) and the F1 fraction inhibited the
P. aeruginosa
biofilm growth above 80% at 4 and 1.3 µg/mL, respectively. These proteins affected the structure of the biofilm, as well as
fle
Q and
fle
R gene expressions involved in the formation and regulation of the
P. aeruginosa
biofilm. The results demonstrated that the F1 fraction proteins of
M. beecheii
honey inhibit and affect the formation of the
P. aeruginosa
biofilm.