Ureibacillus aquaedulcis sp. nov., isolated from freshwater well and reclassification of Lysinibacillus yapensis and Lysinibacillus antri as Ureibacillus yapensis comb. nov. and Ureibacillus antri comb. nov

• Published in: Archives of microbiology Vol. 206; no. 5; p. 242
• Main Authors: Yadav, Amit, Teware, Ruchi, Bhatt, Agrima, Bhavsar, Yash, Maurya, Akanksha, Thorat, Vipool, Vemuluri, Venkata Ramana, Kirdat, Kiran
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-05-2024; Springer Nature B.V
• Subjects: Agricultural land; Amino acids; Bacillaceae - classification; Bacillaceae - genetics; Bacillaceae - isolation & purification; Bacillaceae - metabolism; Bacterial Typing Techniques; Base Composition; Biochemical characteristics; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Catalase; Cell Biology; DNA, Bacterial - genetics; Ecology; Esculin; Fatty Acids - analysis; Fatty Acids - metabolism; Fresh Water - microbiology; Genetic relationship; Life Sciences; Lipids; Lysinibacillus; Microbial Ecology; Microbiology; New species; Original Paper; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids - analysis; Phylogenetics; Phylogeny; Potassium nitrate; Quinones; Reclassification; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Taxonomic revision; Urease; Ureibacillus; Water sampling; Freshwater bacteria; Lysinibacillus; Ureibacillus; Bacillus
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-024-03970-0

A Gram-stain-positive aerobic, rod-shaped, spore-producing bacterium forming colonies with convex elevation and a smooth, intact margin was isolated from a freshwater sample collected from a well situated in an agricultural field. The 16S rRNA gene sequence of the isolated strain BA0131
T
showed the highest sequence similarity to
Lysinibacillus yapensis
ylb-03
T
(99.25%) followed by
Ureibacillus chungkukjangi
2RL3-2
T
(98.91%) and
U. sinduriensis
BLB-1
T
(98.65%). The strain BA0131
T
was oxidase and catalase positive and urease negative. It also tested positive for esculin hydrolysis and reduction of potassium nitrate, unlike its phylogenetically closest relatives. The predominant fatty acids in strain BA0131
T
included were anteiso-C
15:0
, iso-C
16:0
, iso-C
15:0
, iso-C
14:0
and the major polar lipids comprised were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The respiratory quinones identified in strain BA0131
T
were MK8 (H2) (major) and MK8 (minor). The strain BA0131
T
shared the lowest dDDH values with
L. yapensis
ylb-03
T
(21%) followed by
U. chungkukjangi
2RL3-2
T
(24.2%) and
U. sinduriensis
BLB-1
T
(26.4%) suggesting a closer genetic relationship
U. sinduriensis
BLB-1
T
. The ANI percentage supported the close relatedness with
U. sinduriensis
BLB-1
T
(83.61%) followed by
U. chungkukjangi
2RL3-2
T
(82.03%) and
U. yapensis
ylb-03
T
(79.57%). The core genome-based phylogeny constructed using over 13,704 amino acid positions and 92 core genes revealed the distinct phylogenetic position of strain BA0131
T
among the genus
Ureibacillus
. The distinct physiological, biochemical characteristics and genotypic relatedness data indicate the strain BA0131
T
represents a novel species of the genus
Ureibacillus
for which the name
Ureibacillus aquaedulcis
sp. nov. (Type strain, BA0131
T
 = MCC 5284
T
 = JCM 36475
T
) is proposed. Additionally, based on extensive genomic and phylogenetic analyses, we propose reclassification of two species,
L. yapensis
and
L. antri
, as
U. yapensis
comb. nov. (Type strain, ylb-03
T
 = JCM 32871
T
 = MCCC 1A12698
T
) and
U. antri
(Type strain, SYSU K30002
T
 = CGMCC 1.13504
T
 = KCTC 33955
T
).