Effects of allogeneic bone marrow-derived mesenchymal stem cells on T and B lymphocytes from BXSB mice

• Published in: DNA and cell biology Vol. 24; no. 7; p. 458
• Main Authors: Deng, Weimin, Han, Qin, Liao, Lianming, You, Shengguo, Deng, Hongye, Zhao, Robert C H
• Format: Journal Article
• Language: English
• Published: United States 01-07-2005
• Subjects: Animals; B-Lymphocytes - cytology; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Bone Marrow Cells - cytology; CD40 Antigens - metabolism; CD40 Ligand - metabolism; Cell Proliferation - drug effects; Cells, Cultured; Coculture Techniques; Concanavalin A - pharmacology; Disease Models, Animal; Dose-Response Relationship, Drug; Flow Cytometry; Genetic Markers - drug effects; Immunoglobulin G - metabolism; Interferon-gamma - metabolism; Interleukin-4 - metabolism; Lipopolysaccharides - pharmacology; Lupus Erythematosus, Systemic - immunology; Lymphocyte Activation - drug effects; Mesenchymal Stromal Cells - immunology; Mice; Mice, Inbred BALB C; Mice, Mutant Strains; Mitogens - pharmacology; Spleen - cytology; T-Lymphocytes - cytology; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; Transplantation, Homologous - immunology
• ISSN: 1044-5498
• DOI: 10.1089/dna.2005.24.458

Bone marrow-derived mesenchymal stem cells (bMSCs) can differentiate into a number of different cell/tissue types, and also possess immunoregulatory functions. The present study was undertaken to elucidate the exact immunoregulatory effects of allogeneic bMSCs on T- and B-lymphocyte proliferation, activation, and function maturation of BXSB mice, which has been considered as a experimental model for human systemic lupus erythematosus (SLE). We determined that bMSCs from BALB/c mice had inhibitory effects on BXSB mice T-lymphocyte proliferation, but no inhibitory effect on their activation. In addition, they had a significant inhibitory and stimulatory effect on IL-4- and IFN-gamma-producing T cells, respectively. Also, bMSCs had inhibitory effects on the proliferation, activation, and IgG secretion of B lymphocytes. In addition, BALB/c bMSCs had an enhancing effect on CD40 expression and inhibitory effects on CD40 ligand (CD40L) ectopic hyperexpression on B cells from BXSB mice.