Effect of Inducers and Inhibitors of the Keap1/Nrf2/ARE System on the Viability and Functional Activity of Model Neuronal-Like and Glial Cells

• Published in: Bulletin of experimental biology and medicine Vol. 176; no. 3; pp. 332 - 337
• Main Authors: Menshchikova, E. B., Chechushkov, A. V., Kozhin, P. M., Romakh, L. P., Serykh, A. E., Khrapova, M. V., Petrova, E. S., Kandalintseva, N. V.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2024; Springer Nature B.V
• Subjects: Aging; Animals; Antibodies; Antioxidants; Antioxidants - metabolism; Apoptosis; Autophagy; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell lines; Cell viability; Drug dosages; Glial cells; Glioblastoma; Humans; Internal Medicine; Kelch-Like ECH-Associated Protein 1 - genetics; Kelch-Like ECH-Associated Protein 1 - metabolism; Laboratory Medicine; Mice; Neuroblastoma; Neurodegenerative diseases; Neuroglia - metabolism; Neuronal-glial interactions; NF-E2-Related Factor 2 - metabolism; Oxidative Stress; Pathology; Pharmacology and Toxicology; Physiology; Regulatory sequences; Signal Transduction; neurodegenerative diseases; apoptosis; autophagy; proliferation; Keap1/Nrf2/ARE system
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-024-06019-w

On mouse neuroblastoma (Neuro-2a) and human glioblastoma (U-87 MG) cell lines, we studied the effect of inducers and inhibitors of redox-sensitive signaling system of the antioxidant-responsive element Keap1/Nrf2/ARE on the main processes that determine nerve cell viability and vital activity (proliferative activity, apoptosis, autophagy, and activation of the Keap1/Nrf2/ARE system). Inhibitors of the Keap1/Nrf2/ARE system stimulate apoptosis more pronouncedly than inducers, have a weaker effect on autophagy, and do not change the nuclear to cytoplasmic Nrf2 ratio. In general, the revealed effects testify in favor of the potential effectiveness of stimulating the Keap1/Nrf2/ARE system for the prevention and adjuvant therapy of neurodegenerative diseases.