CRISPR-Cas System, Antimicrobial Resistance, and Enterococcus Genus-A Complicated Relationship

CRISPR-Cas System, Antimicrobial Resistance, and Enterococcus Genus-A Complicated Relationship

(1) Background: The rise in antibiotic resistant bacteria poses a significant threat to public health worldwide, necessitating innovative solutions. This study explores the role of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in the context of antibiotic resistance among differ...

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Published in:Biomedicines Vol. 12; no. 7; p. 1625
Main Authors: Costache, Carmen, Colosi, Ioana, Toc, Dan-Alexandru, Daian, Karla, Damacus, David, Botan, Alexandru, Toc, Adelina, Pana, Adrian Gabriel, Panaitescu, Paul, Neculicioiu, Vlad, Schiopu, Pavel, Iordache, Dumitrana, Butiuc-Keul, Anca
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 22-07-2024
Subjects:
Antibiotic resistance
Antibiotics
Antimicrobial resistance
Arrays
Bacteria
Comparative analysis
CRISPR
CRISPRCasFinder
Drug resistance
efmA
Enterococcus
Enterococcus genus
Genes
Genetic engineering
Genomes
Infections
IsaE
Molecular modelling
Online data bases
Plasmids
Public health
Software
Enterococcus faecalis
CRISPR
CRISPRCasFinder
Enterococcus faecium
antibiotic resistance
vanA
efmA
IsaE
Enterococcus genus
vanM
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Summary:(1) Background: The rise in antibiotic resistant bacteria poses a significant threat to public health worldwide, necessitating innovative solutions. This study explores the role of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in the context of antibiotic resistance among different species from the genus. (2) Methods: The genomes of included in the study were analyzed using CRISPRCasFinder to distinguish between CRISPR-positive (level 4 CRISPR) and CRISPR-negative genomes. Antibiotic resistance genes were identified, and a comparative analysis explored potential associations between CRISPR presence and antibiotic resistance profiles in species. (3) Results: Out of ten antibiotic resistance genes found in species, only one, the gene, showed a strong association with CRISPR-negative isolates, while the others did not significantly differ between CRISPR-positive and CRISPR-negative genomes. (4) Conclusion: These findings indicate that the gene may be more prevalent in CRISPR-negative genomes, and they may contribute to a better understanding of the molecular mechanisms underlying the acquisition of antibiotic resistance genes in species.
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ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines12071625