Homogeneity study of the internal quality control sera for immunodiagnosis of HIV/AIDS

• Published in: Jornal brasileiro de patologia e medicina laboratorial Vol. 50; no. 1; pp. 46 - 52
• Main Authors: Castejon, Márcia Jorge, Yamashiro, Rosemeire, Oliveira, Camila Cardoso de, Olivieri, José Carlos, Oliveira, Carmem Aparecida de Freitas, Ueda, Mirthes
• Format: Journal Article
• Language: English
• Published: Sociedade Brasileira de Patologia Clínica; Sociedade Brasileira de Patologia; Sociedade Brasileira de Citopatologia 01-02-2014; Sociedade Brasileira de Patologia Clínica
• Subjects: controle de qualidade; HIV; material de referência; MEDICAL LABORATORY TECHNOLOGY; MEDICINE, RESEARCH & EXPERIMENTAL; PATHOLOGY; técnicas imunoenzimáticas; material de referência; controle de qualidade; HIV; quality control; reference material; técnicas imunoenzimáticas; immunoenzyme techniques
• ISSN: 1676-2444; 1678-4774
• DOI: 10.1590/S1676-24442014000100006

Introduction: The present study reports the data from the first homogeneity assessment of samples composing the serum panels produced at the Immunology Center of Instituto Adolfo Lutz, São Paulo. These samples have been distributed to the public laboratories and those partaking in the Brazilian Unified Health System, and to the participants in the Internal Quality Control Program for human immunodeficiency virus (HIV) antibody (Ab) testing. Objective: To assess the homogeneity of serum samples in panels from different lots for HIV/acquired immunodeficiency syndrome (AIDS) immunodiagnosis by using the statistical method to ensure quality of the reference material. Method: Sera homogeneity was evaluated by means of enzyme-linked immunoassay/enzyme immunoassay (ELISA/EIA) for detection of HIV Ab, and the one-way analysis of variance was employed for analyzing the data. No statistically significant differences were found among the several serum vials. Conclusion: The sera dispensed in the vials were homogeneous in the respective lots.