Simultaneous Determination of Lipophilic Vitamin Esters Using Square‐wave Voltammetry at the Glassy Carbon Electrode

• Published in: Electroanalysis (New York, N.Y.) Vol. 33; no. 2; pp. 537 - 542
• Main Authors: Jashari, Granit, Musliu, Arbër, Sýs, Milan, Arbneshi, Tahir, Mikysek, Tomáš, Švancara, Ivan, Metelka, Radovan
• Format: Journal Article
• Language: English
• Published: 01-02-2021
• Subjects: Cosmetics; Retinyl acetate; Retinyl palmitate; Square-wave voltammetry; α-Tocopheryl acetate
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/elan.202060302

Simple and rapid voltammetric method for simultaneous determination of all‐trans‐retinyl acetate (RAc) or all‐trans‐retinyl palmitate (RPa) and α‐tocopheryl acetate (α‐TOAc) has been proposed. The respective method was based on the anodic oxidation of the compounds of interest by square‐wave voltammetry in acetone with 0.1 mol L−1 LiClO4 at the glassy carbon electrode. The procedure was also beneficial with respect to simple dissolution of sample directly in the supporting electrolyte. The all‐trans‐retinyl acetate could be quantified in two linear ranges (3.1–140 μmol L−1 and 140–400 μmol L−1) and α‐tocopheryl acetate in linear range 5.3–400 μmol L−1 with detection limits of 0.9 μmol L−1 RAc (or 0.8 μmol L−1 RPa) and of 1.6 μmol L−1 α‐TOAc. Selected commercial cosmetic products were analysed achieving satisfactory recoveries.