Rapid one-step in situ synthesis of carbon nanoparticles with cellulosic paper for biosensing

• Published in: Sensors and actuators. B, Chemical Vol. 339; p. 129849
• Main Authors: Chng, Kevin Ziyang, Tan, Justin Kok Soon, Park, Soyeon, Lim, Jiaqi, Chua, Delia Xue Wen, Le, Qiujian, Fang, Jie, Ouyang, Jianyong, Yap, Choon Hwai, Tien, Sim Leng, Leo, Hwa Liang, Kim, Sangho
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15-07-2021; Elsevier Science Ltd
• Subjects: Anemia; Carbon; Carbon nanoparticles; Cellulose; Correlation coefficients; Diameters; Engraving; Hemoglobin; Hemoglobin measurement; Laser engraving; Nanoparticles; Paper-based analytical devices; Synthesis; Laser engraving; Hemoglobin measurement; Carbon nanoparticles; Paper-based analytical devices
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2021.129849

•A carbon nanoparticles-embedded paper-based analytical device (CNP-PAD) is developed to quantify hemoglobin concentration.•The fabrication technique combined the CNP synthesis and embedment processes into a facile single-step procedure.•CNP-PAD uses cellulosic paper as the sole fabrication material.•Synthesized CNP was comprehensively characterized to ascertain its optical property and chemical compositions.•CNP-PAD’s applicability as a biosensor to detect the alterations in hemoglobin (Hb) concentration was demonstrated.
The embedment of carbon nanoparticles (CNPs) on paper-based analytical devices (PADs) possesses great potential as a biosensing platform owing to the synergistic combination of their respective advantageous properties. However, their applicability in actual biomedical scenarios is undermined by their cumbersome fabrication process. Here, we present a CNP-embedded PAD (CNP-PAD) that is fabricated through a one-step laser engraving method. We demonstrate the ultra-fast in situ synthesis (< 20 s) of CNPs (diameter = 23 ± 9 nm) with cellulose paper by simple laser engraving. The CNP-PAD was characterized and optimized for hemoglobin measurements. Validation results obtained with human blood samples demonstrated a high degree of agreement (Pearson correlation coefficient = 0.97) with the standard laboratory measurement approach (cyanmethemoglobin method). This work creates a nexus between the fields of CNP and PAD, presenting advantages that highlight its suitability for accessible anemia diagnostics, particularly in resource-limited environments.