Emerging pathogen Arcobacter spp. in acute gastroenteritis: molecular identification, antibiotic susceptibilities and genotyping of the isolated arcobacters

• Published in: Journal of medical microbiology Vol. 61; no. 10; pp. 1439 - 1444
• Main Authors: KAYMAN, Tuba, ABAY, Seçil, HIZLISOY, Harun, IBRAHIM ATABAY, H, SERDAR DIKER, K, AYDIN, Fuat
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01-10-2012
• Subjects: Adolescent; Adult; Aged; Anti-Bacterial Agents - pharmacology; Arcobacter - classification; Arcobacter - drug effects; Arcobacter - genetics; Arcobacter - isolation & purification; Biological and medical sciences; Child; Child, Preschool; Communicable Diseases, Emerging - epidemiology; Communicable Diseases, Emerging - microbiology; Diarrhea - microbiology; DNA, Bacterial - genetics; Drug Resistance, Bacterial - genetics; Electrophoresis; Feces - microbiology; Female; Fundamental and applied biological sciences. Psychology; Gastroenteritis - microbiology; Genotype; Genotyping Techniques; Gram-Negative Bacterial Infections - epidemiology; Gram-Negative Bacterial Infections - microbiology; Humans; Infectious diseases; Male; Medical sciences; Microbiology; Middle Aged; Polymerase Chain Reaction - methods; Species Specificity; Turkey - epidemiology; Young Adult; Turkey; Antibiotic; Sensitivity; Typing; Acute; Gastroenteritis; Digestive diseases; Intestinal disease; Genotype; Identification; Gastric disease
• ISSN: 0022-2615; 1473-5644
• DOI: 10.1099/jmm.0.044594-0

The aims of this study were as follows: (i) to isolate Arcobacter spp. from the stool samples of patients with gastroenteritis; (ii) to identify them with molecular methods; (iii) to genotype them using enterobacterial repetitive intergenic consensus (ERIC)-PCR; and (iv) to determine their antibiotic susceptibilities. For the study, a total of 3287 diarrhoeal stool samples submitted to the Microbiology Laboratory of the Kayseri Training and Research Hospital, Kayseri, Turkey, between 2010 and 2011 were analysed. Campylobacter blood-free selective medium supplemented with cefoperazone, amphotericin B and teicoplanin was used for isolation. Medium inoculated with stool samples was incubated microaerobically at 37 °C for 72-96 h. Phenotypic tests, a genus-specific PCR and a multiplex PCR were used to identify the arcobacters, whilst ERIC-PCR was used for genotyping and the antibiotic susceptibilities of the isolates were detected by E-test. Arcobacter spp. were isolated from nine of the 3287 samples. These nine isolates were identified as Arcobacter butzleri and all showed different ERIC-PCR profiles. All nine isolates were resistant to ampicillin and susceptible to gentamicin, tetracycline, erythromycin and ciprofloxacin. As far as is known, this is the first study in which A. butzleri has been isolated from human acute gastrointestinal infections in Turkey. According to these results, it is recommended that, when investigating the aetiology of infections of the digestive system in humans, Arcobacter spp. be considered for inclusion. The results of this study should contribute to our knowledge related to A. butzleri infections in humans.