The omission of the “wet” pre‐transplant crossmatch in renal transplant centres in Scotland

• Published in: HLA Vol. 94; no. 1; pp. 3 - 10
• Main Authors: Turner, David, Battle, Richard, Akbarzad‐Yousefi, Arash, Little, Ann‐Margaret
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-07-2019
• Subjects: antibody; Blood Grouping and Crossmatching - methods; Cold Ischemia; crossmatch; Graft Rejection - immunology; histocompatibility; Histocompatibility Testing - methods; HLA; HLA Antigens - immunology; Humans; immunogenetics; Isoantibodies - immunology; kidney; Kidney Transplantation - methods; transplant; virtual; kidney; virtual; antibody; histocompatibility; immunogenetics; transplant; HLA; crossmatch
• ISSN: 2059-2302; 2059-2310
• DOI: 10.1111/tan.13558

The methods used for assessment of immunological risk for a patient receiving a kidney from a deceased donor have undergone significant change in the last few years. Many centres now proceed to transplant without any additional laboratory‐based HLA testing for patients who are well defined as HLA antibody negative. Using rapid HLA antibody tests at the time of donor offer, such as Luminex, it is also possible to omit wet crossmatches in many sensitised patients. This virtual crossmatch (vXM) approach provides benefits in reducing cold ischaemia time (CIT), but also carries risks such as missing clinically relevant non‐HLA reactivity or allelic HLA antibody reactivity. A number of factors need to be in place in a laboratory to enable a vXM policy to be extended to both sensitised and non‐sensitised patients including access to complete donor HLA typing, ability to undertake Luminex‐based HLA antibody testing out of working hours, and access to senior H&I Scientist expertise to assess and interpret results. Other approaches, such as using peripheral blood lymphocytes for crossmatching, may also enable a reduction in CIT and transplant units need to assess the risks of extending vXM processes for their patients against potential benefits.