Bactericidal effect of photodynamic therapy against methicillin-resistant Staphylococcus aureus strain with the use of various porphyrin photosensitizers

• Published in: Acta biochimica polonica Vol. 54; no. 3; pp. 665 - 670
• Main Authors: Grinholc, Mariusz, Szramka, Bozena, Olender, Katarzyna, Graczyk, Alfreda
• Format: Journal Article
• Language: English
• Published: Poland 01-01-2007
• Subjects: Light; Methicillin Resistance; Microbial Viability - drug effects; Microbial Viability - radiation effects; Photochemotherapy - methods; Photosensitizing Agents - chemistry; Photosensitizing Agents - pharmacology; Porphyrins - chemistry; Porphyrins - pharmacology; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - radiation effects
• ISSN: 0001-527X; 1734-154X
• DOI: 10.18388/abp.2007_3240

Photodynamic therapy (PDT) is based on photosensitizers activated by light of appropriate wavelength. Their activation leads to generation of singlet oxygen and free radicals responsible for the cytotoxic effect. The aim of this project was to compare the bactericidal effect of PDT using different porphyrin photosensitizers against a methicillin-resistant Staphylococcus aureus strain. Exogenous sensitizers (protoporphyrin IX and newly synthesized derivative, protoporphyrin diarginate) induced a 3 log10-unit reduction in bacterial viable counts. With the use of endogenous, ALA-induced porphyrins, a 1.6 log10-unit reduction was obtained. The sensitizers tested executed their antibacterial activity with no essential change in the antibiotic resistance pattern of the studied strain.