Endometriosis: Effect of endometriosis on white blood cell subpopulations in peripheral blood and peritoneal fluid of baboons

• Published in: Human reproduction (Oxford) Vol. 11; no. 8; pp. 1736 - 1740
• Main Authors: Hooghe, T.M.D., Hill, J.A., Oosterlynck, D.J., Koninckx, P.R., Bambra, C.S.
• Format: Journal Article
• Language: English
• Published: Oxford University Press 01-08-1996
• Subjects: baboon (Papio); endometriosis; menstrual cycle; peritoneal fluid; white blood cells; endometriosis; ); baboon; peritoneal fluid; white blood cells; menstrual cycle
• ISSN: 0268-1161; 1460-2350
• DOI: 10.1093/oxfordjournals.humrep.a019478

In women with endometriosis, changes in peripheral blood and pentoneal fluid white blood cell (WBC) populations have been reported, but it is not known whether these alterations are causally related to or a consequence of endometriosis. The purpose of this study was to test the hypothesis that peripheral blood and peritoneal fluid WBC populations are altered in baboons with spontaneous and induced endometriosis compared to animals without dis ease. Peripheral blood and peritoneal fluid samples were obtained at laparoscopy from 60 baboons with a normal pelvis (n=23), spontaneous endometriosis (n=19) and induced disease (n=18) during menses (n=9), folllcular phase (n=12), luteal phase (n=20), pregnancy or nursing (n=11) and in non-cycling animals (n=8). The WBC concentration was analysed with a Coulter counter and fluorescent antibody cell separation (FACS) analysis was used to measure cluster designation (CD)2, CD4, CD8, interleukin (IL)2R and leuclne (Leu) M5 subsets. In peripheral blood, the percentage of CD4+ and IL2R+ cells was increased in baboons with stage II-IY spontaneous or induced endometriosis, suggesting that alterations in peripheral blood WBC populations may be an effect of endometriosis. In peritoneal fluid the WBC concentration and percentages of Leu M5+ macrophages and CD8+ lymphocytes were only increased in baboons with spon taneous endometriosis and not in animals with induced disease, suggesting that alterations in peritoneal fluid WBC populations may lead to the development of endometriosis. In summary, the results of this study suggest that peripheral blood and pentoneal fluid immune cell populations are affected in baboons with endometriosis.