Recombinant human alpha-fetoprotein as a regulator of adipose tissue stromal cell activity

• Published in: Russian journal of bioorganic chemistry Vol. 38; no. 5; pp. 459 - 468
• Main Authors: Zubkova, E. S., Semenkova, L. N., Dudich, I. V., Dudich, E. I., Khromykh, L. M., Makarevich, P. I., Parfyonova, Y. V., Menshikov, M. Yu
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01-09-2012
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Life Sciences; Organic Chemistry; migration; recombinant human alpha-fetoprotein; angiogenic factors; internalization; adipose tissue-derived stromal cells; cell therapy
• ISSN: 1068-1620; 1608-330X
• DOI: 10.1134/S1068162012050147

Recombinant human alpha-fetoprotein (rhAFP), expressed in the yeast system as a glycoprotein, has been isolated and purified to 98% by the multistep method. The testing of rhAFP in the culture of adipose tissue stromal cells (hASC) has revealed its ability to enhance hASC proliferation and migration, as well as the vascular endothelial growth factor production, with no reliable influence on cell invasion and secretion of matrix metalloproteinases-2 and -9. It has also been established that rhAFP is internalized in hASC via a clathrin-dependent mechanism. The study in the murine experimental model of hindlimb ischemia has shown the ability of rhAFP to enhance blood flow recovery. These data suggest that rhAFP is a promising agent for enhancement of the hASC regenerative ability.