Sustainable PVP-Capped Silver Nanoparticles as a Free-Standing Nanozyme Sensor for Visual and Spectrophotometric Detection of Hg2+ in Water Samples: A Green Analytical Method

• Published in: Chemosensors Vol. 10; no. 9; p. 358
• Main Authors: Abdel-Lateef, Mohamed A., Almahri, Albandary, Alzahrani, Eman, Pashameah, Rami Adel, Abu-Hassan, Ahmed A., El Hamd, Mohamed A.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-09-2022
• Subjects: Accuracy; Calcium ions; Carbon dioxide; Cobalt; Color fading; Environmental impact; Enzymes; Food contamination & poisoning; Hydrogen peroxide; Magnesium; mercury (II); Mercury (metal); Microwaves; Nanomaterials; Nanoparticles; O-phenylenediamine; Peroxidase; peroxidase-like activity; Phenylenediamine; Polydispersity; Polyvinylpyrrolidone; PVP-AgNPs; Scientific imaging; Selectivity; Sensors; Signal transduction; Silver; silver nanoparticles; Silver nitrate; Sodium citrate; Spectrophotometry; Trivalent ions; Visible spectrum; water samples; Water sampling; United Kingdom > UK; Japan
• ISSN: 2227-9040; 2227-9040
• DOI: 10.3390/chemosensors10090358

In the proposed method, microwave-assist heating and AgNO3/trisodium citrate were used to create the polyvinylpyrrolidone-capped silver nanoparticles (PVP-AgNPs) sensor. This sensor had a peroxidase-like activity that could catalytically oxidize O-phenylenediamine (OPD, colourless) into 2,3-diaminophenazine (ox-OPD, greenish-yellow colour) in the presence of H2O2, otherwise, in the presence of Hg2+, this pass has been effectively inhibited. The degree of colour fading was directly correlated with Hg2+ concentration. These results indicated the selectivity of Hg2+ ions toward PVP-AgNPs after establishing the PVP-AgNPs/OPD/H2O2 system. This selectivity was proved by the negative results obtained from other mon-, di-, and trivalent ions such as Na+, K+, Ca2+, Mg2+, Ba2+, Co2+, Ni2+, Cd2+, and Cr3+, instead of Hg2+. Consequently, a reliable, selective, and eco-effective spectrophotometric approach was designed for the detection of Hg2+ in various types of water samples. LOD was extended to lower than 0.1 µM, and a fading in the obtained colour was shown by the naked eye at a concentration higher than 1.5 µM of Hg2+. The elemental details for preparing the used PVP-AgNPs, such as particle size, morphology, polydispersity index (PdI), and their UV-visible spectrum, were identified by SEM technique, TEM, UV-visible spectrophotometer, and zeta-sizer device. Thus, the peroxidase mimicking the activity of OPD/H2O2 was confirmed by a fluorescence technique. The greenness profile of this work was confirmed after applying a reported assessment tool.