Recombinant destabilase from the medicinal leech: Preparation and properties
The procedure for obtaining an active recombinant destabilase from the medicinal leech in Escherichia coli cells was developed. The plasmids encoding an analogue of native destabilase, as well as the protein forms carrying polyhistidine sequence at the Cand/or N-terminus of the polypeptide were obta...
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Published in: | Russian journal of bioorganic chemistry Vol. 42; no. 1; pp. 42 - 52 |
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Main Authors: | , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Moscow
Pleiades Publishing
2016
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Subjects: | |
Online Access: | Get full text |
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Summary: | The procedure for obtaining an active recombinant destabilase from the medicinal leech in
Escherichia coli
cells was developed. The plasmids encoding an analogue of native destabilase, as well as the protein forms carrying polyhistidine sequence at the Cand/or N-terminus of the polypeptide were obtained during the work. The producing strains of different forms of the protein were constructed, the cultivation process was optimized. The conditions of renaturation of destabilase recombinant forms by dialysis and using chromatographic absorbent were selected. The muramidase activity towards cell walls of
Micrococcus lysodeikticus
bacferia and lytic activity towards
E. coli
were investigated. The dependence of pH and ionic strength of the solution on the activities was determined. The total antibacterial activity of destabilase towards
E. coli
was shown. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1068-1620 1608-330X |
DOI: | 10.1134/S1068162016010088 |