Molecular detection of multidrug and colistin-resistant Escherichia coli isolated from house flies in various environmental settings

• Published in: Future microbiology Vol. 14; no. 10; pp. 847 - 858
• Main Authors: Sobur, Abdus, Haque, Zobayda F, Sabuj, Abdullah Am, Ievy, Samina, Rahman, Amm Taufiqur, El Zowalaty, Mohamed E, Rahman, Tanvir
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01-07-2019
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Bacteria; Colistin; Colistin - pharmacology; Dairy farms; Dairy products; Diptera - microbiology; Drug resistance; Drug Resistance, Multiple, Bacterial - genetics; E coli; Environmental Monitoring; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli - pathogenicity; Farms; Food; Hospitals; Microbial Sensitivity Tests; Microorganisms; Multidrug resistance; Muscidae; Pathogens; Poultry; Public health; Salmonella; Virulence; Virulence - genetics; United States > US; Bangladesh; China; antibiotic; one-health; epidemiology; environment; virulence; colistin; house flies; multidrug
• ISSN: 1746-0913; 1746-0921
• DOI: 10.2217/fmb-2019-0053

To detect antibiotic-resistant pathogenic
in house flies captured from various environmental settings.
Isolation and identification of
was performed based on culture and PCR. Antimicrobial susceptibility testing was conducted using disk diffusion test. Detection of virulence and antimicrobial resistance genes was carried out using PCR methods.
The prevalence of
in flies was 61% with highest in dairy farms (70.67%) followed by food centers (65.33%), dustbins (64%) and area close to hospital (44%). The prevalence rates of
and
resistance determinants were 88.75, 48.52, 41.67, 37.17, 27.77, 18.37 and 14.81%, respectively.
Multidrug-resistant pathogenic
are widely distributed in house flies, which requires a one-health approach to delineate their control.