Focal modulation microscopy: a theoretical study

Focal modulation microscopy is an emerging fluorescence microscopy technique for in vivo imaging of thick biological tissues. Here, we present a theoretical study to assess its performance. The scalar diffraction theory is combined with Monte Carlo simulation to evaluate the signal-to-background rat...

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Bibliographic Details
Published in:	Optics letters Vol. 35; no. 11; p. 1804
Main Authors:	Chong, Shau Poh, Wong, Chee Howe, Sheppard, Colin J R, Chen, Nanguang
Format:	Journal Article
Language:	English
Published:	United States 01-06-2010
Subjects:	Algorithms Image Enhancement - methods Image Interpretation, Computer-Assisted - methods Imaging, Three-Dimensional - methods Microscopy, Fluorescence - methods Reproducibility of Results Sensitivity and Specificity
Online Access:	Get more information
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Summary:	Focal modulation microscopy is an emerging fluorescence microscopy technique for in vivo imaging of thick biological tissues. Here, we present a theoretical study to assess its performance. The scalar diffraction theory is combined with Monte Carlo simulation to evaluate the signal-to-background ratio at various depths. The performance of confocal microscopy with a similar optical setup is also evaluated for comparison.
ISSN:	1539-4794
DOI:	10.1364/OL.35.001804