Impact of litter Salmonella status during feed withdrawal on Salmonella recovery from the broiler crop and ceca

• Published in: Poultry science Vol. 96; no. 12; pp. 4361 - 4369
• Main Authors: Buhr, R J, Bourassa, D V, Hinton, Jr, A, Fairchild, B D, Ritz, C W
• Format: Journal Article
• Language: English
• Published: England 01-12-2017
• Subjects: Animal Feed - analysis; Animals; Cecum - microbiology; Chickens; Crop, Avian - microbiology; Environmental Microbiology; Floors and Floorcoverings; Food Deprivation; Male; Poultry Diseases - microbiology; Salmonella enterica - classification; Salmonella enterica - physiology; Salmonella Infections, Animal - microbiology; Serogroup; Salmonella; litter; ceca; crop; broiler
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps/pex231

Research was conducted to evaluate the impact of litter Salmonella status during feed withdrawal on Salmonella recovery from the crop and ceca following feed withdrawal. In 4 experiments, pens of broilers in separate rooms were challenged with marker strains of either Salmonella Montevideo or Salmonella Heidelberg. Three d post challenge, a 12-hour feed withdrawal was initiated, and one pen of broilers was switched between rooms for each Salmonella serotype. In experiments 3 and 4, non-challenged broilers also were added to the Salmonella challenge pens. The litter of each pen was sampled before and after the feed withdrawal period, the broilers euthanized, and the crop and ceca aseptically removed for Salmonella isolation. Results showed that only the challenge Salmonella serotype was recovered from the litter in challenge pens where broilers were not moved, while both Salmonella serotypes were recovered from the litter of the switched pens. Salmonella was recovered from 56/80 crops and from 66/80 ceca of challenged broilers that remained in the challenge pens. The challenge Salmonella serotype was recovered from 50/80 crops and from 60/80 ceca, and the switched pens' litter Salmonella serotype was recovered from 19/80 crops but not from the ceca in broilers challenged with Salmonella and then switched between pens. For experiments 3 and 4, Salmonella was recovered from 19/40 crops and from only 2/40 ceca from the non-challenged broilers placed into the Salmonella challenge pens. The results from broilers that were switched between Salmonella challenge pens indicate that the recovery of Salmonella from the crop of broilers following feed withdrawal (on Salmonella-contaminated litter) appears to depend mainly on the initial challenge Salmonella (62%) and less on the litter Salmonella (24%) status during the feed withdrawal period. In contrast, only the initial challenge Salmonella was recovered from the ceca (79%) from broilers that remained in challenge pens or were switched between Salmonella challenge pens. However, when non-challenged broilers were placed into the Salmonella challenge pens and commingled during the 12-hour feed and water withdrawal period, it was possible to recover the pen litter Salmonella from the ceca at a low level of 5% (2/40).