A FRET-based probe with a chemically deactivatable quencher

A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a chemical reagent (sodium dithionite). This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted pr...

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Bibliographic Details
Published in:	Chemical communications (Cambridge, England) Vol. 48; no. 26; p. 3224
Main Authors:	Leriche, Geoffray, Budin, Ghyslain, Darwich, Zeinab, Weltin, Denis, Mély, Yves, Klymchenko, Andrey S, Wagner, Alain
Format:	Journal Article
Language:	English
Published:	England 01-01-2012
Subjects:	Caspase 3 - chemistry Caspase 3 - metabolism Dithionite - chemistry Fluorescence Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemistry HeLa Cells Humans Molecular Structure Recombinant Proteins - chemistry Recombinant Proteins - metabolism
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Summary:	A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a chemical reagent (sodium dithionite). This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted probes in cell experiments.
ISSN:	1364-548X
DOI:	10.1039/c2cc17542h