Signaling function of phagocytic NADPH oxidase: Activation of MAP kinase cascades in phagocytosis

• Published in: Russian journal of bioorganic chemistry Vol. 36; no. 1; pp. 124 - 129
• Main Authors: Markvicheva, K. N., Gorokhovatskii, A. Yu, Mishina, N. M., Mudrik, N. N., Vinokurov, L. M., Luk’yanov, S. A., Belousov, V. V.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 2010
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Experimental Articles; Life Sciences; Organic Chemistry; Erk1/2; phagocytosis; NADPH oxidase; hydrogen peroxide
• ISSN: 1068-1620; 1608-330X
• DOI: 10.1134/S1068162010010140

Until recently, the production of reactive oxygen species by NADPH oxidase has been considered only in the context of the oxidative damage to pathogens inside the phagosome. However, homologues of phagocytic NADPH oxidase have been found in almost all cell types, where they produce hydrogen peroxide and thereby regulate the initial intracellular stages of MAP kinase cascades. In the present work, the activation of two MAP kinase cascades, p38 and Erk1/2, during phagocytosis has been studied. It was found that phagocytosis activates both cascades. The activation of Erk1/2 is dependent, and the activation of p38 is not dependent, on the activity of NADPH oxidase. Therefore, the activation of MAP kinases in phagocytes during phagocytosis occurs by a mechanism similar to that operating in nonphagocytic cells, indicating the universality of the function of NADPH oxidases in different cell types.