Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits

• Published in: Semina. Ciências agrárias : revista cultural e científica da Universidade Estadual de Londrina Vol. 39; no. 5; pp. 1993 - 2000
• Main Authors: Pinto Filho, Saulo Tadeu Lemos, Rosa, Matheus Pippi da, Treichel, Tiago Luis Eilers, Dalmolin, Fabíola, Machado, Alencar Kolinski, Cadoná, Francine Carla, Cruz, Ivana Beatrice Mânica da
• Format: Journal Article
• Language: English
• Published: Universidade Estadual de Londrina 20-09-2018
• Subjects: CTM; ERO; Oryctolagus cuniculus; Terapia celular
• ISSN: 1676-546X; 1679-0359
• DOI: 10.5433/1679-0359.2018v39n5p1993

Mesenchymal stem cells are a population of somatic cells found in several tissues of an adult organism, including adipose tissue. Reactive oxygen species (ROS) can cause cellular alterations, including mutagenesis and genomic instability and the development of diseases. Thus, it is important to understand ROS-induced damage to cell macromolecules such as DNA, proteins, and lipids. In this study, we investigated oxidative stress rates and viability of adipose tissue-derived mesenchymal stem cells (ADSCs) from the greater omentum of rabbits. Cell cultures were analyzed at different passages (1-5) using the dichlorofluorescein acetate assay for measuring ROS production and cell viability tests. ROS levels were highest at passage 2 and cell viability was highest at passage 4.