Lack of association of a common polymorphism in the 3' -UTR of interleukin 8 with non small cell lung cancer in Kashmir

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 14; no. 7; pp. 4403 - 4408
• Main Authors: Bhat, Imtiyaz Ahmad, Pandith, Arshid A, Bhat, Bashir A, Naykoo, Niyaz A, Qasim, Iqbal, Rasool, Roohi, Aziz, Sheik Aejaz, Shah, Zafar Amin
• Format: Journal Article
• Language: English
• Published: Thailand 01-01-2013
• Subjects: 3' Untranslated Regions - genetics; Adenocarcinoma - genetics; Adenocarcinoma - pathology; Adenocarcinoma, Bronchiolo-Alveolar - genetics; Adenocarcinoma, Bronchiolo-Alveolar - pathology; Carcinoma, Large Cell - genetics; Carcinoma, Large Cell - pathology; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Case-Control Studies; Female; Follow-Up Studies; Genotype; Humans; India; Interleukin-8 - genetics; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Male; Middle Aged; Neoplasm Staging; Polymerase Chain Reaction; Polymorphism, Genetic - genetics; Polymorphism, Restriction Fragment Length; Prognosis; India
• ISSN: 1513-7368; 2476-762X
• DOI: 10.7314/APJCP.2013.14.7.4403

Chronic inflammation is considered as an important factor in the pathogenesis of lung cancer. The presence of inflammatory cells and higher levels of pro-inflammatory cytokines in the tumor microenvironment and their surrounding tissues is gaining much importance in research.
One hundred ninety NSCLC cases and 200 age, smoking and sex matched controls were evaluated for association of IL-8 -251 (rs4073) and IL-8 -845 (rs2227532) in our population. Restriction fragment length polymorphism (RFLP) was used followed by direct sequencing for the detection of SNPs.
The IL-8 -845 polymorphism was not found in our population. No significant association was observed between the IL-8 -251 AT genotypes and IL-8 -25 AA genotypes and NSCLC (p=0.05) in our population. The IL-8 -251 A allele was also non-significant (p=0.05) in NSCLC patients.
In conclusion, this report reveals lack of association between IL-8 - 251 A/T polymorphism and NSCLC in our Kashmir Valley population.