Determination of biofilm formation, antibiotic susceptibility profiles and quorum sensing mediated virulence factors in ceftazidime resistant Pseudomonas aeruginosa

• Published in: Biológia Vol. 78; no. 10; pp. 2881 - 2893
• Main Authors: Başkan, Ceren, Yıldırım, Tuba, Bilgin, Melek, Sivas, Hülya
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-10-2023; Springer Nature B.V
• Subjects: Antibiotic resistance; Antibiotics; Biofilms; Biomedical and Life Sciences; Ceftazidime; Cell Biology; Drug resistance; Elastase; Life Sciences; Microbiology; Original Article; Pathogenesis; Plant Sciences; Polymerase chain reaction; Pseudomonas aeruginosa; Pyocyanin; Quorum sensing; Resistance factors; RhlR protein; Virulence; Virulence factors; Zoology; Biofilm; Virulence factors; Quorum sensing
• ISSN: 1336-9563; 0006-3088; 1336-9563
• DOI: 10.1007/s11756-023-01429-z

This study aims to investigate the relationship between the antibiotic resistance, biofilm production, virulence factors, and quorum sensing systems of
Pseudomonas aeruginosa
. First, disc diffusion and microdilution tests were applied to determine the antibiotic susceptibility of isolates. Second, virulence factors of isolates were determined phenotypically. Then, intact and internal
lasI
,
lasR
,
rhII
, and
rhIR
gene regions were amplified by polymerase chain reaction. Finally, biofilm formed by isolates were observed using a confocal laser scanning microscope. As a result, significant differences were found between hospital services and antibiotic resistance (
p
 < 0.05). Biofilm formation was observed in 76% of the isolates. The production of homoserine lactone, pyocyanin, and elastase were detected in 51%, 65%, 66% of isolates, respectively. The
lasI
gene was 96% intact and 95% internal, and the
lasR
was 91% intact and 95% internal for the las system. The
rhlI
was 91% intact and 90% internal, and the
rhlR
was 80% intact and 90% internal for the rhl system. According to the microscope results, the isolates with antibiotic resistance formed thicker biofilm layer. Biofilm-positive strains were capable of important virulence factors and developed antibiotic resistance, playing a role in the pathogenesis.