Non-genomic action of vitamin D3 on N-methyl-D-aspartate and kainate receptor-mediated actions in juvenile gonadotrophin-releasing hormone neurons

• Published in: Reproduction fertility and development Vol. 29; no. 6; p. 1231
• Main Authors: Bhattarai, Pravin, Bhattarai, Janardhan P, Kim, Min Sun, Han, Seong Kyu
• Format: Journal Article
• Language: English
• Published: Australia 01-06-2017
• Subjects: Animals; Cholecalciferol - metabolism; Epigenesis, Genetic; Female; Gonadotropin-Releasing Hormone - secretion; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; In Vitro Techniques; Kainic Acid - metabolism; Male; Mice, Transgenic; N-Methylaspartate - metabolism; Nerve Tissue Proteins - agonists; Nerve Tissue Proteins - antagonists & inhibitors; Nerve Tissue Proteins - metabolism; Neurogenesis; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; Neurons - secretion; Patch-Clamp Techniques; Preoptic Area - cytology; Preoptic Area - drug effects; Preoptic Area - metabolism; Preoptic Area - secretion; Receptors, Kainic Acid - agonists; Receptors, Kainic Acid - antagonists & inhibitors; Receptors, Kainic Acid - metabolism; Receptors, N-Methyl-D-Aspartate - agonists; Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors; Receptors, N-Methyl-D-Aspartate - metabolism; Sodium Channel Blockers - pharmacology; Synaptic Potentials - drug effects
• ISSN: 1031-3613
• DOI: 10.1071/RD15357

Vitamin D is a versatile signalling molecule that plays a critical role in calcium homeostasis. There are several studies showing the genomic action of vitamin D in the control of reproduction; however, the quick non-genomic action of vitamin D at the hypothalamic level is not well understood. Therefore, to investigate the effect of vitamin D on juvenile gonadotrophin-releasing hormone (GnRH) neurons, excitatory neurotransmitter receptor agonists N-methyl-D-aspartate (NMDA, 30μM) and kainate (10μM) were applied in the absence or in the presence of vitamin D3 (VitaD3, 10nM). The NMDA-mediated responses were decreased by VitaD3 in the absence and in the presence of tetrodotoxin (TTX), a sodium-channel blocker, with the mean relative inward current being 0.56±0.07 and 0.66±0.07 (P<0.05), respectively. In addition, VitaD3 induced a decrease in the frequency of gamma-aminobutyric acid mediated (GABAergic) spontaneous postsynaptic currents and spontaneous postsynaptic currents induced by NMDA application with a mean relative frequency of 0.595±0.07 and 0.56±0.09, respectively. Further, VitaD3 decreased the kainate-induced inward currents in the absence and in the presence of TTX with a relative inward current of 0.64±0.06 and 0.68±0.06, respectively (P<0.05). These results suggest that VitaD3 has a non-genomic action and partially inhibits the NMDA and kainate receptor-mediated actions of GnRH neurons, suggesting that VitaD3 may regulate the hypothalamic-pituitary-gonadal (HPG) axis at the time of pubertal development.