Dose-dependent effect of H2O2 on hairy roots of Scutellaria baicalensis: growth, composition of fatty acids, and flavones

• Published in: Plant cell, tissue and organ culture Vol. 155; no. 3; pp. 893 - 905
• Main Authors: Solov’eva, A. I., Stepanova, A. Y., Ivanova, T. V., Voronkov, A. S., Panov, Yu. M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-12-2023; Springer Nature B.V
• Subjects: Aglycones; Biomedical and Life Sciences; Composition; Fatty acids; Flavones; Hairy root; Herbal medicine; Hydrogen peroxide; Life Sciences; Lipids; Medicinal plants; Molecular chains; Original Article; Oxidative stress; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Reactive oxygen species; Roots; Scutellaria baicalensis; Oxidative stress; Wogonin; Baicalein; Unsaturated index; Very-long-chain fatty acid; Wogonoside; Baicalin
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-023-02609-0

Scutellaria baicalensis
Georgi. is a valuable medicinal plant. The hairy roots strain of
S. baicalensis
(Sc. baic.-1) from IPP RAS collection was used as a model system for the study. Effects of 1 and 10 mM of hydrogen peroxide (H
2
O
2
) were first reported on the growth characteristics of hairy roots, fatty acid (FAs) composition of total lipids, and major root-specific flavones during the cultivation cycle. H
2
O
2
at a concentration of 1 mM exhibited the properties of a signaling molecule: the total FAs content of total lipids increased mainly due to polyunsaturated FAs, including very-long-chain FAs (VLCFAs); the total flavones content was about 1.5-fold higher than in the control. Besides, at 1 mM H
2
O
2
the content of aglycones, especially wogonin, was higher than in the other variants. At 10 mM H
2
O
2
, the properties of H
2
O
2
as reactive oxygen species were manifested: inhibition in the growth of hairy root culture was observed against the background of an increase in the content of malondialdehyde; the proportion of saturated FAs and the total proportion of VLCFAs increased; the amount of the aglycone baicalein was also significantly reduced. Based on the data obtained, we can speak about a possible role of VLCFAs in the protection of
S. baicalensis
roots against oxidative stress.
Key message
Hydrogen peroxide (H
2
O
2
) has a dose-dependent effect on the hairy roots of
Scutellaria baicalensis
, 1 mM H
2
O
2
stimulates the synthesis of flavones, 10 mM H
2
O
2
increases the absolute amount of fatty acids of lipids, but in both cases increases the content of very-long-chain fatty acids.