Usage of 3-methyl-1-β-cyclodextrinimidazole tosylate for electrophoretic separation and preconcentration of corticosteroids by capillary electrophoresis

• Published in: Monatshefte für Chemie Vol. 152; no. 9; pp. 1067 - 1074
• Main Authors: Kravchenko, Anastasia V., Kolobova, Ekaterina A., Kartsova, Liudmila A.
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 2021; Springer Nature B.V
• Subjects: Analytical Chemistry; Capillary electrophoresis; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chloroform; Corticosteroids; Cortisone; Cyclodextrins; Electrokinetics; Electrophoresis; Hormones; Hydrocortisone; Inorganic Chemistry; Organic Chemistry; Original Paper; Physical Chemistry; Steroids; Theoretical and Computational Chemistry; Ionic liquids; Cyclodextrins; On-line preconcentration; Macrocycles; Capillary electrophoresis; Steroids
• ISSN: 0026-9247; 1434-4475
• DOI: 10.1007/s00706-021-02809-4

Nowadays, there are plenty of approaches to determine steroids hormones by capillary electrophoresis, however, the development of new ones is still actual. In the present study, the possibilities of 3-methyl-1-β-cyclodextrinimidazole tosylate as pseudo stationary phase are considered. Due to the formation of positively charged complexes between steroids with a derivative based on β-cyclodextrin and imidazolium cation, the following results were achieved: first, it was established that adding of this modifier at concentration 0.70 mM at pH 3.2 allowed to separate the five most important corticosteroid hormones (cortisone, hydrocortisone, 11-deoxycortisol, corticosterone, 21-hydroxyprogesterone). Second, the presence of a modifier in the sample permitted to carry out electrokinetic injection of samples and preconcentration of analytes under field-amplified sample injection mode. Obtained values of limits of detection (5–15 ng/cm
3
) allowed to analyze plasma samples after liquid–liquid extraction by chloroform.
Graphic abstract