Endophytic rhizobia promote the growth of Cuban rice cultivar

• Published in: Symbiosis (Philadelphia, Pa.) Vol. 85; no. 2; pp. 175 - 190
• Main Authors: Hernández, Ionel, Taulé, Cecilia, Pérez-Pérez, Reneé, Battistoni, Federico, Fabiano, Elena, Rivero, Deyanira, Nápoles, María Caridad
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-11-2021; Springer Nature B.V
• Subjects: Bacteria; Biofilms; Biomedical and Life Sciences; Confocal microscopy; Cultivars; Developmental Biology; Ecology; Endophytes; Evolutionary Biology; Grasslands; Inoculation; Legumes; Life Sciences; Microbiology; Phylogeny; Plant bacterial diseases; Plant Sciences; Rhizobium; Rhizosphere; Rice; rRNA 16S; Siderophores; Strains (organisms); Plant growth promotion; Endophytes; Rice
• ISSN: 0334-5114; 1878-7665
• DOI: 10.1007/s13199-021-00803-2

Bacteria from the rhizobia group have been previously identified as grass-endophytes in grassland with cropping history of legumes. In this paper we aimed to demonstrate that strains of the
Rhizobium
genus, isolated from the rhizosphere of a rice Cuban cultivar subjected to intensive monoculture management, behave as endophytes and promote its growth. Eleven bacteria previously isolated from the rhizosphere of rice plants, were screened for their plant growth promoting traits. We found that some of them were able to solubilize inorganic phosphate compounds, produce siderophores and biofilm, have exocellulase and protease activity and were able to inhibit the phytopathogen
Pyricularia oryzae
growth. The phylogenetic analysis using partial sequences of
16S rRNA
gene showed that bacteria isolated from rice plant of cultivar INCA LP-5 belonged to
Rhizobium
,
Agrobacterium
and
Pseudomonas
genera. Two strains analyzed,
Rhizobium
sp. Rpr2 and Rdp16, increased the height and biomass of rice plants, 70 days post-inoculation in greenhouse conditions. According to the quantification of
Rhizobium
sp. strain Rpd16 in surface-disinfected tissues of root and sheath, together with the analysis of confocal microscopy images, we can conclude that the Rdp16 strain is a rice endophyte of the cultivar INCA LP-5. This investigation is the first evidence of endophytic rhizobia able to promote the rice growth of a Cuban cultivar subjected to intensive monoculture, without rotation with legume plants and without a previous history of rhizobia inoculation.