Transcript Profiles Differentiate Cold Acclimation-Induced Processes in a Summer and Winter Biotype of Camelina

• Published in: Plant molecular biology reporter Vol. 40; no. 2; pp. 359 - 375
• Main Authors: Wang, Hongxia, Doğramacı, Münevver, Anderson, James V., Horvath, David P., Chao, Wun S.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-06-2022; Springer Nature B.V
• Subjects: Acclimation; Acclimatization; Bioinformatics; Biological clocks; Biomedical and Life Sciences; Biosynthesis; Biotypes; Camelina; Circadian rhythms; Cold; Cold acclimation; Cold tolerance; Cold treatment; Flowering; Freezing; Genes; Life Sciences; Lipids; Metabolomics; MicroRNAs; miRNA; Oilseed crops; Oilseeds; Original Article; Photosynthesis; Plant Breeding/Biotechnology; Plant Sciences; Proteomics; Ribonucleic acid; RNA; Summer; Transcriptomes; Vernalization; Winter; Camelina; RNAseq; Vernalization; Cold acclimation
• ISSN: 0735-9640; 1572-9818
• DOI: 10.1007/s11105-021-01324-4

Camelina (
Camelina sativa
L. Crantz) is a short-season oilseed crop of the
Brassicaceae
family that consists of both summer and winter annual biotypes. Winter biotypes require non-freezing cold conditions for acquiring freezing tolerance (cold acclimation) and floral initiation (vernalization). Transcriptome profiles of a summer (CO46) biotype with poor freezing tolerance after acclimation and a winter (Joelle) biotype with excellent freezing tolerance after acclimation were compared prior to and after an 8-week cold treatment to identify key molecular pathways and genes responsive to cold acclimation and vernalization and potentially associated with freezing tolerance. Gene-set enrichment analyses identified AraCyc pathways involved in photosynthesis and lipid and hormone biosynthesis that were different between the two biotypes. Sub-network enrichment analyses identified hubs of molecular networks such as circadian clock, flowering, and hormone and stress responsive genes that were likely involved in vernalization but may also overlap with cold-induced freezing tolerance. A microRNA involved in floral initiation (MIR172A) was identified as a central hub for microRNA targets among upregulated genes for Joelle post-acclimation. Combined results are generally consistent with many previously identified molecular pathways and genes acting together to control vernalization, cold acclimation, and freezing tolerance. Our research provides new insights into the regulation of cold acclimation and molecular genetic mechanisms underlying cold tolerance and floral induction for the winter biotype Joelle.