Environmental enrichment enhances ethanol preference over social reward in male swiss mice: Involvement of oxytocin-dopamine interactions

• Published in: Neuropharmacology Vol. 253; p. 109971
• Main Authors: Rae, Mariana, Gomes, Ivone, Spelta, Lidia Emmanuela Wiazowski, Bailey, Alexis, Marcourakis, Tania, Devi, Lakshmi, Camarini, Rosana
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-08-2024
• Subjects: Carbetocin; Motivation; Nucleus accumbens; Phospholipase C; Social dominance; Striatum; Nucleus accumbens; Striatum; Carbetocin; Social dominance; Motivation; Phospholipase C
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/j.neuropharm.2024.109971

The impact of environmental enrichment (EE) on natural rewards, including social and appetitive rewards, was investigated in male Swiss mice. EE, known for providing animals with various stimuli, was assessed for its effects on conditioned place preference (CPP) associated with ethanol and social stimuli. We previously demonstrated that EE increased the levels of the prosocial neuropeptide oxytocin (OT) in the hypothalamus and enhanced ethanol rewarding effects via an oxytocinergic mechanism. This study also investigated the impact of EE on social dominance and motivation for rewards, measured OT-mediated phospholipase C (PLC) activity in striatal membranes, and assessed OT expression in the hypothalamus. The role of dopamine in motivating rewards was considered, along with the interaction between OT and D1 receptors (DR) in the nucleus accumbens (NAc). Results showed that EE mice exhibited a preference for ethanol reward over social reward, a pattern replicated by the OT analogue Carbetocin. EE mice demonstrated increased social dominance and reduced motivation for appetitive taste stimuli. Higher OT mRNA levels in the hypothalamus were followed by diminished OT receptor (OTR) signaling activity in the striatum of EE mice. Additionally, EE mice displayed elevated D1R expression, which was attenuated by the OTR antagonist (L-368-889). The findings underscore the reinforcing effect of EE on ethanol and social rewards through an oxytocinergic mechanism. Nonetheless, they suggest that mechanisms other than the prosocial effect of EE may contribute to the ethanol pro-rewarding effect of EE and Carbetocin. They also point towards an OT-dopamine interaction potentially underlying some of these effects.
•Environmental Enrichment (EE) and Carbetocin increase the preference for ethanol reward over social reward.•EE increases oxytocin (OT) mRNA levels in the hypothalamus.•EE diminishes PLC activity in the striatum.•EE promotes social dominance and reduces reward motivation.•EE mechanisms involve OT-D1R interactions in NAc.